In vitro Shoot Induction and Growth of Black Galangal (Alpinia nigra (Gaertn.) Burtt)
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Abstract
The majority of Black galangal (A. nigra (Gaertn.) Burtt) propagation occurs through rhizome separation, and distribution by seeds in natural conditions. Black galangal belonging to the genus Alpinia is used as a food plant related to human life, medicinal plant, and ornamental plant. Propagation of Black galangal using tissue culture techniques can quickly increase the number of plants for commercial use and genetic conservation. The objective of this study was to investigate in vitro shoot induction and shoot growth of black galangal. Black galangal seeds were sterilized with Clorox solution at concentrations 10 and 5 percent for about 15 and 10 minutes, respectively. Seeds were germinated on Murashige and Skoog (MS). In vitro shoots of black galangal were cultured on MS medium supplemented with various concentrations of BA (0, 0.5, 1.0 and 1.5 mg/L). The results showed that the MS medium supplemented with 0.5, 1.0 and 1.5 mg/L BA had the highest number of shoots (2.20, 2.70 and 2.70, respectively). The highest root and root length were found on MS medium without plant growth regulators. Therefore, the propagation of Black galangal for germplasm conservation
and commercial use can be cultured on MS medium supplemented with 0.5 - 1.5 mg/l BA.
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References
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