Partial Nucleotide Sequencing of rbcL Gene of Nutmeg Herb (Myristica fragans Houtt.)

Main Article Content

Chawanrat Somnuek
Phisut Kanboon

Abstract

Nutmeg (Myristica fragans Houtt.) is an herb, which is widely grown in tropical rain forests, especially in the eastern and the southern parts of Thailand. Almost every part of nutmeg could be utilized not only in the household as a culinary spice, flavoring agent but medical benefits as well. However, the study of nutmeg genetic information has been insufficient. Therefore, the nutmeg samples were used to investigate the nucleotide sequence of the rbcL gene in this research. The results revealed that the 932 bp of the amplified rbcL gene showed similarity with the dicotyledons in the nutmeg family (Myristicaceae). The nucleotide sequences showed the highest identity with Knema sp. (Accession number AB586432.1 and AB586431.1) and Myristica fragrans (Accession number AY298839.1). It could be inferred from this study that the locus of rbcL gene can be used for amplification and plant genetic relationships study.

Article Details

How to Cite
Somnuek, C., & Kanboon, P. (2020). Partial Nucleotide Sequencing of rbcL Gene of Nutmeg Herb (Myristica fragans Houtt.). YRU Journal of Science and Technology, 5(1), 34–40. retrieved from https://li01.tci-thaijo.org/index.php/yru_jst/article/view/235312
Section
Research Article

References

Bussadee, P., Thanananta, T. & Thanananta, N. (2015). Identification of Mangoes Cultivars in Thailand using

DNA Sequences of rpoC1 and rbcL Genes. Thai Science and Technology Journal, 23(6)

(Special Issue), 983-993. (in Thai)

Herbal Medicines Database, Faculty of Pharmacy, Ubon Ratchathani University. (n.d.) Dok Chan. [online] Retrieved December 6, 2019, from: http://www.thaicrudedrug.com/main.php?action=viewpage&pid=55 (in Thai)

Honestdocs. (2019). Nutmeg benefits, eating ideas and using nutmeg for health and caution. [online] Retrieved December 6, 2019, from: https://www.honestdocs.co/the-benefits-of-nutmeg (in Thai)

Honey J., Araya K.R., Sindhu T.U., Syamjith P., Vinood K.R. & Sandhya, S. (2016). A Descriptive Review on Myristica fragans Houtt. Hygeia: Journal of Drugs and Medicines, 8(1), 35 – 43.

Jaiswal, P., Kumar, P., Singh, V.K. & Singh, D.K. (2009). “Biological Effects of Myristica fragrans”, ARBS Annual Review of Biomedical Sciences, 11, 21-29.

Jangid, K., Jayakumar, N.D. & Varghese, S.S. (2014). Achievable Therapeutic Effects of Myristica Fragrans

(Nutmeg) on Periodontitis a Short Review. International Journal of Pharmacy and Pharmaceutical

Sciences, 6(5), 591 – 594.

Keereekoch, T., Srisuwan, T. & Sangkaew, N. (2018). Quality Comparison of Nutmeg from Thailand and

Indonesia. Thaksin University Journal, 21(2), 33 – 42. (in Thai)

Maya, K.M., Zachariah, T.J., Krishnamoorthy, B. (2004). Chemical composition of essential oil of nutmeg

(Myristica fragrans Houtt.) accessions. Journal of spice and aromatic crops, 13, 135-139.

Orwa, C., Mutua, A., Kindt, R., Jamnadass, R. & Simons, A. (2009). Agroforestree Database: a tree reference and selection guide version 4.0. World Agroforestry Centre, Kenya. [online] Retrieved December 9, 2019, from: https://www.worldagroforestry.org/resources/databases/agroforestree.

Plunkett, G.M., Soltis, D.E. and Soltis, P.S. (1997). Clarification of the relationship between Apiaceae and Araliaceae based on matK and rbcL sequence data. American Journal of Botany, 84, 565-580.

Prommanee, W. (2017). DNA Barcoding of Plants in the Genus Solanum L. in Thailand. Thesis of

Master of Science (Pharmaceutical Sciences) Graduate School, Silpakorn University. (in Thai)

Rozen, S & Skaletsky, H. (2000). Primer3 on the WWW for General Users and for Biologist Programmers, In Krawetz, S. and Misener, S. (eds). Bioinformatics Methods and Protocols: Methods in Molecular Biology, Humana Press, Totowa, New Jersey, 365-386.

Sonavane, G.S., Palekar R.C., Kasture V.S. & Kasture, S.B. (2001). Behavioral actions of Myristica fragrans seeds. Indian Journal of Pharmacology, 33, 417 – 424.

Tan, K.P., Khoo, H.E. & Azrina, A. 2013. Comparison of antioxidant components and antioxidant capacity in

different parts of nutmeg (Myristica fragrans). Food Research International, 20, 1049-1052.

Thanananta, N., Saetai, K. & Thanananta, T. (2014a). Genetic Relationship Analysis of Bulbophyllum

Section Sestochilus using Nucleotide Sequences of Specific Genes. Thai Science and Technology

Journal, 22(4), 523-530. (in Thai)

Thanananta, N., Sumrith, J. & Thanananta, T. (2014b). Analysis of Genetic Relationship of Colored Rice

Cultivars Using DNA Sequences of rbcL and rpoC1 Genes. Thai Science and Technology Journal,

(5)(Special Issue), 674 – 682. (in Thai)

Traditional Home Remedies 2013. (2013, 14 February). Government Gazette. Chapter 130 Special Issue

, 30-49.