The Optimal Concentration of Coconut Water on Multiple Shoot Induction of Musa (AAA group) ‘Kluai Nak’ In Vitro
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Abstract
Red bananas (Musa (AAA group) ‘Kluai Nak’) are rare in Thailand and it is at risk of extinction because of environmental changes and human behavior. Hence, plant tissue culture technique is used for propagation. The optimal concentration of coconut water on multiple shoot of red banana in vitro was studied. Shoot explants were cultured on Murashige and Skoog (MS) medium supplemented with coconut water at 4 different concentrations and subcultured at monthly intervals for 3 months. The experimental design was Completely Randomized Design (CRD). The results indicated that MS medium supplemented with 150 mL/L coconut water gave the highest average number of shoots at 8.25 shoots/culture shoot and the highest average shoot length at 7.73 cm which were significantly different (p<0.01) from the other concentrations.
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