The efficacy of herbal extract to control onion twister disease causal by Collectotrichum gloeosporioides fungal pathogen

Main Article Content

Wimonsiri Sehawong
Sayan Subepang
Suvichark Aroonluk

Abstract

The efficacy of extracts from 5 herbs was studied as follows garlic, fingerroot, galangal, turmeric and lemongrass, was studied in inhibiting Colletotrichum gloeosporioides. which is causative agent of the disease in shallot. Tested with Poison Food Technique, extracted with 95% ethanol. The culture medium containing 1,000 ppm of extracts was incubated at 25 °C for 3, 5, 7 and 9 days. Measure diameter of the fungi on the culture medium, was found that extracts of fingerroot, garlic, galangal, lemongrass, turmeric and control. Found that ability to inhibit the growth of Colletotrichum gloeosporioides were as follows: 93.86, 70.56, 24.56, 17.79, 15.79 and 0 percent, respectively. Field test to study the percentage of shallot incidence index. There were 5 test methods: M1) uninoculated, sprayed with water M2) inoculated, sprayed with water M3) inoculated, sprayed with fingerroot extract M4) inoculated with benomyl sprayed, M5) inoculated with benomyl and fingerroot sprayed, was found that M4 had a disease index of 2.17 percent. M3 had a disease incidence index of 11 percent and M5 had a disease incidence index of 7.50%. This experiment efficacy of fingerroot in controlling Colletotrichum gloeosporioides disease in shallots. Benefit to reduce use of chemicals and reduce development of fungi that affect fungal resistance.

Article Details

How to Cite
Sehawong, W., Subepang, S., & Aroonluk, S. (2022). The efficacy of herbal extract to control onion twister disease causal by Collectotrichum gloeosporioides fungal pathogen. YRU Journal of Science and Technology, 7(2), 35–42. retrieved from https://li01.tci-thaijo.org/index.php/yru_jst/article/view/252261
Section
Research Article
Author Biographies

Wimonsiri Sehawong, Program in Agricultural Technology, Faculty of Arts and Sciences, Sisaket Rajabhat University

The efficacy of extracts from five herbs including garlic, fingerroot, galangal, turmeric, and lemongrass in controlling Colletotrichum gloeosporioides caused disease in shallot was examined using Poison Food Technique. The extracts were carried out using 95percent ethanol. Each extract at concentration of 1,000 mg/L was added in fungal culture and incubated at 25 °C for 9 days. The results show that extracts of fingerroot could inhibit growth of fungi the best at 93.86% significantly different (P<0.05) with another treatments. For field experiment the results revealed that application of Benomyl gave the lowest disease index at 2.17 followed by application with Benomyl alternate by fingerroot extract which gave disease index at 7.50 significantly different (P<0.05) with another treatments. Thus, fingerroot extract is an alternative choice to apply in controlling C. gloeosporioides disease in shallots both in laboratory scale and field conditions for farmers in order to avoid the use of chemicals.

Sayan Subepang, Program in Agricultural Technology, Faculty of Arts and Sciences, Sisaket Rajabhat University

The efficacy of extracts from five herbs including garlic, fingerroot, galangal, turmeric, and lemongrass in controlling Colletotrichum gloeosporioides caused disease in shallot was examined using Poison Food Technique. The extracts were carried out using 95percent ethanol. Each extract at concentration of 1,000 mg/L was added in fungal culture and incubated at 25 °C for 9 days. The results show that extracts of fingerroot could inhibit growth of fungi the best at 93.86% significantly different (P<0.05) with another treatments. For field experiment the results revealed that application of Benomyl gave the lowest disease index at 2.17 followed by application with Benomyl alternate by fingerroot extract which gave disease index at 7.50 significantly different (P<0.05) with another treatments. Thus, fingerroot extract is an alternative choice to apply in controlling C. gloeosporioides disease in shallots both in laboratory scale and field conditions for farmers in order to avoid the use of chemicals.

Suvichark Aroonluk, Program in Agriculture, Faculty of Agriculture and life Sciences, Chandrakasem Rajabhat University

The efficacy of extracts from five herbs including garlic, fingerroot, galangal, turmeric, and lemongrass in controlling Colletotrichum gloeosporioides caused disease in shallot was examined using Poison Food Technique. The extracts were carried out using 95percent ethanol. Each extract at concentration of 1,000 mg/L was added in fungal culture and incubated at 25 °C for 9 days. The results show that extracts of fingerroot could inhibit growth of fungi the best at 93.86% significantly different (P<0.05) with another treatments. For field experiment the results revealed that application of Benomyl gave the lowest disease index at 2.17 followed by application with Benomyl alternate by fingerroot extract which gave disease index at 7.50 significantly different (P<0.05) with another treatments. Thus, fingerroot extract is an alternative choice to apply in controlling C. gloeosporioides disease in shallots both in laboratory scale and field conditions for farmers in order to avoid the use of chemicals.

References

Chaiwat, J. & Araya, J. (1999). The Fungicidal Properties of Extracts of Clove (Eugenia caryophyllus Spreng.) and Sweet Flag (Acorus calamus Linn.). Acta Hortic, 501, 87-94.

Elaissi, A., Rouis, Z., Salem, N.A., Mabrouk, S., Bensalem, Y. & Salah, K.B. (2012). Chemical composition of 8 eucalyptus species essential oils and the evaluation of their antibacterial, antifungal and antiviral activities. BMC Complementary Alternative Medicine, DOI: 10.1186/1472-6882-12-81.

Euis, N., Rizal, S., Zainal, A., Sobir, S. & Machfud, M. (2020). Analysis of mancozeb and carbofuran pesticides residues in the production area of shallot (Allium cepa L. var. ascalonicum) Bulgarian. Journal of Agricultural Science, 26(5), 974-981.

Jae, W.H., Sang, H.S., Kyoung S.J., Yong, H.C., Quang, L.D., Hun, K. & Gyung, J.C. (2018). In vivo assessment of plant extracts for control of plant diseases: A sesquiterpene ketolactone isolated from Curcuma zedoaria suppresses wheat leaf rust. Journal of Environmental Science and Health, 53(2), 135-140.

Jin, Z., Xiaoyu, D., Yechun, X., Yongqiang, D., Meiqin, L. & Yao, Z. (2020). Structure of Mpro from SARS-CoV-2 and discovery of its inhibitors. Nature, 528, 289-293.

Jyoti, S., Bhatnagar, S.K. & Akash T. (2019). Study on fungicidal effect of plant extracts on plant pathogenic fungi and the economy of extract preparation and efficacy in comparison to synthetic/chemical fungicides. Journal of Applied and Natural Science, 11(2), 333-337.

Khan, S.A., Zia, K., Ashraf, S., Uddin, R. & Ul-Haq, Z. (2020). Identifcation of chymotrypsin-like protease inhibitors of SARSCoV-2 via integrated computational approach. Journal of Biomolecular Structure and Dynamics, 39(7), 2607-2616.

Kittika, J., Amnart, P., Kanjana, I., Saranya, P., Phanwimol, T. & Susumu, K. (2017). Inhibitory Effects of Thai Essential Oils on Potentially Aflatoxigenic Aspergillus parasiticus and Aspergillus flavus. Biocontrol Science, 22(1), 31-40.

Manasi, K.B. & Ajit G.D. (2014). Antifungal activity of herbal extracts against plant pathogenic fungi. Archives of Phytopathology and Plant Protection, 47(8), 959-965.

Mani, J.S., Janice, S., Mani, J.B., Johnson, J.C., Steel, D.A., Broszczak, P.M., Neilsen, K.B. & Walsh, M.N. (2020). Natural product-derived phytochemicals as potential agents against coronaviruses. Virus Research, 284, 1-16.

Meenakshi, S. & Saurabh, K. (2015). Colletotrichum gloeosporioides: An Anthracnose Causing Pathogen of Fruits and Vegetables. Biosciences Biotechnology Research ASIA, 12(2), 1,233-1,246.

Miyara, C., Shnaiderman, X., Meng, X., Vargas, W.A., Diaz-Minguez, J.M. & Sherman, A. (2012). Role of Nitrogen Metabolism Genes Expressed During Pathogenicity of the Alkalinizing Colletotrichum gloeosporioides and Their Differential Expression in Acidifying Pathogens. The American Phytopathological Society, 25(9), 51-63.

Nuchnualrat, P.& Boonroj, A. (2008). Effect of Prochloraz, Benomyl, Carbendazim, Azoxystrobin, Mancozeb and Copper oxychloride for Controlling Anthracnose Disease of Dragon Fruit. Rajabhat Rambhai Barni Research Journal, 9(2),15-20.

Nuchnualrat, P.& Jitsartta, N. (2022). Evaluation of fungicide carbendazim resistance in Colletotrichum gloeosporioides causing anthracnose disease of mango cv. Aokrong in Chanthaburi province. RMUTSB Academic Journal, 9(2), 164-173

Oranun, O. & Wannee, J. (2017). Fingerroot, Boesenbergia rotunda and its Aphrodisiac Activity. Pharmacogn Rev., 11(21), 27-30.

Phukang, J. & Chaisuk, P. (2016). Effects of some Fungicides on Growth Inhibition of Colletotrichum gloeosporioides, Causal Agent of Onion Twister Disease. Jourual of Agriculture, 32(2), 181-189. (in Thai).

Rattanakreetakul, C. (2013). Fumigation with Plant Volatile Oils to Control Stem End Rot of Banana. Acta Hortic, 973, 207-213