Efficacy of Endophytic Bacteria Formulation for Control Canker Disease of Pomelo cv. ‘Tup Tim Siam’ Caused by Xanthomonas citri subsp. citri in the Field Conditions
Article Sidebar
Main Article Content
Abstract
The canker disease of pomelo cv. ‘Tup Tim Siam’ caused by Xanthomonas citri subsp. citri, which an important disease resulting serious economic effects. The research aims to formulate and study on control the canker disease by 12 formulas of the endophytic bacteria Bacillus subtilis S1-4 in the field conditions. The formula composed of B. subtilis S1-4, carrier substances and performance enhancers in different ratios. The results showed that the best water soluble formula was kaolin, sugar, SiO2, CMC, CaCO3 and FeSO4 at the ratio 75 : 10 : 5 : 5 : 3 : 2 which the highest number of bacteria survival in this formula at 2.0 x 1010 cfu/mL at room temperature for nine months storage. The formula effectively inhibited the growth of X. citri subsp. citri at 1.62 cm compared with Ampicillin by the paper disc diffusion method, a significant difference (P<0.05). To control Tup Tim Siam pomelo canker in the field by B. subtilis S1-4 formula was intra-plant injected and sprayed on pomelo leaves for 5 times every ten days. The results showed 60.20% inhibited X. citri subsp. citri by intra-plant injection, canker lesions at 5.46 lesions/leaf, and 0.38 cm width of yellow area surrounding wound. Spraying method had 69.20% of X. citri subsp. citri inhibition 5.41 canker lesions/leaf and 0.49 cm width of the yellow area surrounding the wound with non-significantly different (P>0.05) when compared with chemical, antibiotic, B. subtilis product from market, and control (untreated).
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
บทความ ข้อมูล เนื้อหา รูปภาพ ฯลฯ ที่ได้รับการเผยแพร่ในวารสารวิทยาศาสตร์และเทคโนโลยี มรย. นี้ ถือเป็นลิขสิทธิ์ของวารสารวิทยาศาสตร์และเทคโนโลยี มรย. หากบุคคลหรือหน่วยงานใดต้องการนำทั้งหมดหรือส่วนหนึ่งส่วนใดไปเผยแพร่ต่อหรือกระทำการใดๆ จะต้องได้รับอนุญาตเป็นลายลักษณ์อักษรจากวารสารวิทยาศาสตร์และเทคโนโลยี มรย. ก่อนเท่านั้น
References
Bolivar-Anillo, H. J., Gonzalez-Rodriguez, V. E., Almeida, G. R., Izquierdo-Bueno, I., Moraga, J., Carbu, M., et al. (2021). Endophyte microorganisms as an alternative for the biocontrol of Phytophthora spp. In Agro-Economic Risks of Phytophthora and a Effective Biocontrol Approach. [Online]. Retrieved March 1, 2023, from: http://dx.doi.org/10.5772/intechopen99696.
Cho, A. T., & Kasem, S. (2018). Modified growth media of Bacillus amyloliquefaciens S20A1 and biocontrol of bacterial leaf blight of rice. Thai Journal of Agricultural Science, 51(4), 195-207.
De Almeida Lopes, K. B., Pipolo, V. C., Fira, D., Balatti, P. A., Lopez, S. M. Y., Oro, T. H., Pagliosa, E. S. & Degrassi, G. (2018). Screening of bacterial endophytes as potential biocontrol agents against soybean disease. Journal of Applied Microbiology, 125, 1466-1481.
Nikaji, J. (2016). Development of formulation and application of Bacillus subtilis for controlling soft rot disease of chinese mustard. Master’s Thesis. Suranaree University of Technology. (in Thai)
Pimpanuwal, P. (2021). Investigating the potential of actinobacteria to prevent disease and promoting the growth of citrus. Master’s Thesis. Naresuan University. (in Thai)
Poralokanon, P., & Athinuwat, D. (2018). Pilot scale product of novel biopesticide wettable powder formulation to control canker disease of lime in large field application. Thai Journal of Science and Technology, 7(5), 491-515. (in Thai)
Prathuangwong, S., Athinuwat, D., Chuaboon, W., Chatnaparat, T., & Buensanteai, N. (2013). Bioformulation Pseudomonas fluorescens SP007s against dirty panicle disease of rice. African Journal Microbiology Research, 7(47), 5274-5283.
Preecha, C., Wisutthiphaet, W., & Seephueak, P. (2018). Occurrence of canker caused by Xanthomonas axonopodis pv. citri on pummelo (Citrus maxima (Burm.) Merr.) cultivar. Tabtim Siam in Nakhon Si Thammarat province, Thailand and screening fungicides, antibiotics and antagonistic bacteria against X. a. pv. citri in vitro. Journal of Geoscience and Environment Protection, 6(3), 1-7.
Qian, J., Zhang, T., Tang, S., Zhou, L., Li, K., Fu, X., & Yu, S. (2021). Biocontrol of citrus canker with endophyte Bacillus amyloliquefaciens QC-Y. Plant Protection Science, 57(1), 1-13.
Rabbee, M. F., Ali, M. S., & Baek, K. H. (2019). Endophyte Bacillus velezensis isolated from Citrus spp. control streptomycin resistant Xanthomonas citri subsp. citri that causes citrus bacterial canker. Agronomy, 9(8), 470. [Online]. Retrieved March 1, 2023, from: http://doi.org/10.3390agronomy 9080470.
Rabbee, M. F., Hwang, B. S., & Baek, K. H. (2023). Bacillus velezensis: a beneficial biocontrol agent or facultative phytopathogen for sustainable agriculture. Agronomy, 13(3), 840. [Online]. Retrieved May 2, 2023, from: https://doi.org/10.3390/agronomy13030840.
Sudyoung, N., Tokuyama, S., Krajangsang, S., Pringsulaka, O., & Sarawaneeyaruk, S. (2020). Bacterial antagonists and their cell-free culture efficient suppress canker disease in citrus lime. Journal of Plant Disease and Protection, 127(2), 173-181.
