Assessment of Chemical Residues, Heavy Metals, and Antioxidant Activity in Marigold Flowers Grown in Betong District, Yala Province, and Development of Cosmetic Products from Marigold Flowers
Main Article Content
Abstract
The marigold flower contains a variety of bioactive compounds, particularly with antioxidant properties. Utilizing downgraded marigold flowers during the production process and converting them into cosmetic products is an interesting option for adding value to local raw materials. The aim of this research was to investigate the total phenolic content and antioxidant properties of graded and downgraded marigold flowers from Betong District, Yala Province, Thailand, for the development of cosmetic products. The analysis of residual chemicals and heavy metals in the soil and marigold flowers revealed that no residual chemicals were found in either the soil or the flowers. Additionally, the levels of heavy metals were within the standard limits. Subsequently, both the graded and downgraded marigold flowers were extracted by maceration with ethanol as the solvent. The total phenolic compounds and the antioxidant activity were evaluated. The results showed that the total phenolic compounds in the crude marigold flower extract are higher than in the graded marigold flowers, with a value of 4.521±0.064 mg GAE/g extract, and an antioxidant activity (IC50) of 0.819±0.042 mg/ml, which is significantly different from the graded marigold flowers. Furthermore, the basic properties of liquid body wash and shampoo products containing marigold flower extract were examined, revealing a pH value between 5 and 6, good stability, no separation, no microorganisms or fungi, and good flow ability. Therefore, it can be concluded that downgraded marigold flowers can be developed into cosmetic products, which adds value and provides an alternative source of income for farmers.
Article Details

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
บทความ ข้อมูล เนื้อหา รูปภาพ ฯลฯ ที่ได้รับการเผยแพร่ในวารสารวิทยาศาสตร์และเทคโนโลยี มรย. นี้ ถือเป็นลิขสิทธิ์ของวารสารวิทยาศาสตร์และเทคโนโลยี มรย. หากบุคคลหรือหน่วยงานใดต้องการนำทั้งหมดหรือส่วนหนึ่งส่วนใดไปเผยแพร่ต่อหรือกระทำการใดๆ จะต้องได้รับอนุญาตเป็นลายลักษณ์อักษรจากวารสารวิทยาศาสตร์และเทคโนโลยี มรย. ก่อนเท่านั้น
References
Anastassiade, M., Lehotay, S.J., Stajbaher, D. & Schenck, F. J. (2003) Fast and easy multiresidues employing actonitrile extraction/Paritioning and "dispersive solid-Phase extraction" for determination of pesticide residues in produce. J. AOAC, Int., 86, 412-431.
Announcement of the National Environmental Board. (2021). Determine soil quality standards. Rachakitcha, 138(54)F, 20-24.
Announcement of the Ministry of Public Health. (2021). Standard, purity value or other characteristics important to the quality of registered herbal product formulas, notify details or register. Rachakitcha, 138(294)F, 6-7.
Apilux, A., Isarankura-Na-Ayudthaya, C., Tantimongcolwat, T. & Prachayasittikul, V.. (2015). Paper-based acetylcholinesterase inhibition assay combining a wetsystem for organophsphate and carbamate pesticides detection. EXCLI Journal, 14, 307-319.
Burlec, A. F., Pecio, Ł., Kozachok, S., Mircea, C., Corciovă, A., Vereștiuc, L. et al. (2021). Phytochemical profile, antioxidant activity, and cytotoxicity assessment of Tagetes erecta L. flowers. Molecules, 26(5), 1201.
Chaowuttikul, C., & Phoomphong, C. (2024). The content of to the ash, acid-insoluble ash and pesticide residue detection contained of Zingiberaceae crude drugs in Bangkok. Research Journal Phranakhon Rajabhat: Science and Technology, 19(1), 29-40. (in Thai)
Chompoo, J., Songklang, W., Ariyamongkonchai, N. & Boonruangrod, R. (2021). Performance of ethanolic extracts from Tagetes patula flowers on antioxidants and against skin aging-related enzymes. Songklanakarin Journal of Plant Science, 8(1), 50-58. (in Thai)
Chuenyen, B., Ratee, U., Polsri, C., K. P. & Khanngern, N. (2023). Factor analysis for cause related marketing, value creation and consumers decision on natural cosmetic products. Journal of Management and Development Ubon Ratchathani Rajabhat University, 10(1), 67-85. (in Thai)
Land Development Department. (1990). Soil chemical analysis process (1st ed.). Work Manual, 25. (in Thai)
Lateh, L. & Muhamad, N. (2023). Effect of microwave-assisted extraction of β-carotene from Borassus flabellifer Fruit in different solvents for cosmetic application. Health Science, Science and Technology Reviews, 16(3), 40–52. (in Thai)
Manivannan, A., Narasegowda, S. & Prakash, T. (2021). Comparative study on color coordinates, phenolics, flavonoids, carotenoids, and antioxidant potential of marigold (Tagetes sp.) with diverse colored petals. Journal of Food Measurement and Characterization, 15, 4343-4353.
Michalak, M. (2022). Plant-derived antioxidants: Significance in skin health and the ageing process. International Journal of Molecular Sciences, 23(2), 585.
Madanan, M. T., Shah, I. K., Varghese, G. K. & Kaushal, R. K. (2021). Application of Aztec Marigold (Tagetes erecta L.) for phytoremediation of heavy metal polluted lateritic soil. Environmental Chemistry and Ecotoxicology, 3, 17-22.
Youssef, H. A., Ali, S. M., Sanad, M. I. & Dawood, D. H. (2020). Chemical investigation of flavonoid, phenolic acids composition and antioxidant activity of Tagetes erecta flowers. Egyptian Journal of Chemistry, 63(7), 2605-2615.
Merdana, I. M., Nandita, I. L., Elia, P., Apsari, I. A. P., Gorda, I. W. & Sudimartini, L. M. (2024). Phytochemical test and antioxidant activity of aqueous extract of Marigold flower. Advances in Tropical Biodiversity and Environmental Sciences, 8(2), 101–107.
Muhamad, N., Soontornnon S. P., Tansom, U., Haron, W., Lateh, L., Kongsrichan, L., et al. (2024). Phytochemical compounds and antioxidant activities of Parkia speciosa Hassk. Journal of Advanced Development in Engineering and Science, 14(39), 132–140. (in Thai)
Office of Royal Development Projects Board (RDPB). (2021). Projects initiated by royal initiative [Online]. Retrieved November 16, 2024, from: https://projects.rdpb.go.th/projects/5974753735081984/-. (in Thai)
Rahim, N. A., Roslan, M. N. F., Muhamad, M. & Seeni, A. (2022). Antioxidant activity, total phenolic and flavonoid content and LC–MS profiling of leaves extracts of Alstonia angustiloba. Separations, 9(9),234.
Rivas-García, L., Crespo-Antolín, L., Forbes-Hernández, T. Y., Romero-Márquez, J. M., Navarro-Hortal, M. D., Arredondo, M., et al. (2023). Bioactive properties of Tagetes erecta edible flowers: Polyphenol and antioxidant characterization and therapeutic activity against ovarian tumoral cells and caenorhabditis elegans tauopathy. International Journal of Molecular Sciences, 25(1), 280.
Sapkaew, U., Namwong, P., Suthanukool, P. & Choodee, K. (2023). Capability enhancement in the production of high-quality Gotu Kola for medicinal plants with nontoxic and heavy metal contamination. Journal of Agricultural Science and Management, 6(1), 69-78. (in Thai)
Saenprakob, P., Wiangkaew, C. & Intana, P. (2023). Shampoo and lotion development containg rambutan seed oil. Research Journal Phranakhon Rajabhat: Science and Technology, 18(1), 84-93. (in Thai)
Singh, Y., Gupta, A. & Kannojia, P. (2020). Tagetes erecta (Marigold)-A review on its phytochemical and medicinal properties. Current Medical and Drug Research, 4(1), 1-6.
Somkhow, P., Junlatat, J., Chalomphong, N., Prasert, T. & Mulateeka, P. (2022). The efficacy of acne gel containing Allium ascalonicum L. extracts. Journal of Traditional Thai Medical Research, 8(2), 99–110. (in Thai)
Son, H. T. & Limanan, D. (2022). Antioxidant capacity test, total phenolic, total alkaloid, and toxicity of marigold flower (Tagetes Erecta L.). Devotion, 4(1), 88–95.
Srithupthai, K. (2018). Detection of pesticide residues in herbal: A case study of farmers group in Ton Phueng sub-district, Phang Khon district, Sakon Nakhon province. Khon Kaen Agriculture Journal, 46(Suppl.1), 841-846. (in Thai)
Thaysnit, N. & Wachirawongsakorn, P. (2021). Assessment of some heavy metal contamination in paddy soils of Sukhothai province. PSRU Journal of Science and Technology, 6(1), 99-108. (in Thai)
Tubtim, P., Chanapaitoon, N. P. & Boonyuen, S. (2018). Development of liquid soap containing the extract of Alium cepa Linn. Thai Science and Technology Journal, 26(4), 545-550.
Wattanasoontorn, P. & Amornsahguansin, J. (2017). Detections of pesticide and herbicides residues in soils samples from paddy fields in Chainat province. Academic Journal Uttaradit Rajabhat University, 11(2), 245–258. (in Thai)
Wisungre, S., Chumanee, S., Duenngai, K., Deechan, S., Paratang, P., Mechai, N., et al. (2023). Development of herbal liquid soap from Areca catechu L. extract toward the community product standard. Journal of Science and Technology Buriram Rajabhat University, 7(1), 47–58. (in Thai)
Yotsawate, S., Phuekvilai, P. & Yookongkaew, N. (2017). Potential of heavy metal uptake and accumulation in dominant herbaceous plants around gold mine areas in Pichit Province. Science and Technology, 25(1), 110-123. (in Thai)