Comparisons of Mycelial Growth of Oyster Mushroom (Pleurotus spp.) on Culture Media and Sorghum Grain Spawn in Relation to Yield from Commercial Spawn Sources in Thailand
Main Article Content
Abstract
Oyster mushroom (Pleurotus spp.) is widely cultivated for commercial purposes. However, genetic variation among strains from different production sources in Thailand leads to differences in yield performance. This study aimed to compare the mycelial growth and yield potential of oyster mushroom strains from 10 commercial sources in Thailand. Mycelial growth was evaluated on PDA medium, sorghum grain, and rubberwood sawdust substrate by recording colony diameter on PDA and sorghum grain, mycelial length on the substrate, mycelial dry weight, and mycelial density. Yield performance was assessed based on fresh mushroom weight and the number of fruiting bodies per cluster. The experiment was conducted using a completely randomized design (CRD), and means were compared using Duncan’s Multiple Range Test (DMRT). The results showed that strains from DOA and WTN MF-RYG exhibited the highest colony diameters on PDA (87.43 mm and 86.87 mm, respectively). On sorghum grain, the DOA strain showed the highest mycelial growth (96.77 mm). In terms of dry weight, strains from DOA, SP MF-CMI, SRY MF-LEI, and SHP-TAK were among the highest performers (10.02–5.99 g). Regarding yield, strains from DD MF-SKA, SP MF-CMI, SRY MF-LEI, SHP-TAK, WTN MF-RYG, NKP LM-NPT, PP MF-PCT, and CB MF-RBR produced high fresh weights (67.42–56.57 g) and a large number of fruiting bodies per cluster (19.67–9.33 mushrooms/cluster). Overall, the DOA strain demonstrated the best performance across all parameters. Additionally, strains from SHP-TAK, SRY MF-LEI, and SP MF-CMI exhibited rapid mycelial colonization on the substrate and good yield potential, making them suitable for commercial cultivation. These findings provide practical guidance for selecting and developing oyster mushroom strains that meet the specific needs of commercial producers.
Article Details

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
บทความ ข้อมูล เนื้อหา รูปภาพ ฯลฯ ที่ได้รับการเผยแพร่ในวารสารวิทยาศาสตร์และเทคโนโลยี มรย. นี้ ถือเป็นลิขสิทธิ์ของวารสารวิทยาศาสตร์และเทคโนโลยี มรย. หากบุคคลหรือหน่วยงานใดต้องการนำทั้งหมดหรือส่วนหนึ่งส่วนใดไปเผยแพร่ต่อหรือกระทำการใดๆ จะต้องได้รับอนุญาตเป็นลายลักษณ์อักษรจากวารสารวิทยาศาสตร์และเทคโนโลยี มรย. ก่อนเท่านั้น
References
Abdullah, N., Ismail, R., Johari, N. M. K. & Annuar, M. S. M. (2013). Production of liquid spawn of an edible grey oyster mushroom, Pleurotus pulmonarius (Fr.) Quél by submerged fermentation and sporophore yield on rubber wood sawdust. Scientia Horticulturae, 161, 65-69.
Adebayo, E. A., Elkanah, F. A., Afolabi, F. J., Ogundum, O. S., Alabi, T. F. & Oduoye, O. T. (2021). Molecular characterization of most cultivated Pleurotus species in sub-western region Nigeria with development of cost-effective cultivation protocol on palm oil waste. Helion, 7, 1-8.
Aditya, b., Neeraj, n., Jarial, R. S., Jarial, K. & Bhatia, J. N. (2024). Comprehensive review on oyster mushroom species (Agaricomycetes): Morphology, nutrition, cultivation and future aspects. Heliyon, 10(5), 1-28.
Ahmed, A. F., Mahamed, G. A., Hefzy, M., Liu, Z. & Ma, C. (2023). Overview on the edible mushroom in Egypt. Journal of Future Food, 3(1), 8-15.
Alvarez, L. V. & Bautista, A. B. (2021). Growth and yield performance of Pleurotus on selected lignocellulosic wastes in the vicinity of PUP main campus, Philippines. Indian Journal of Science and Technology, 14(3), 259-269.
Bellettini, M. B., Fiorda, F. A., Maieves, H. A., Teixeira, G. L., Ávila, S., Hornung, P. S, et al. (2019). Review: Factors affecting mushroom Pleurotus spp. Saudi Journal of Biological Sciences, 26, 633-646.
Devi, P. V., Ialam, J., Narzary, P., Sharma, D. & Sultana, F. (2024). Bioactive compounds, nutraceutical values and its application in food product development of oyster mushroom. Journal of Future Foods, 4(4), 335-342.
Lee, A. M. L., Chin, C. F. S., Seelan, J. S. S., Chye, F. Y., Lee, H. H. & Rakib, M. R. M. (2023). Metabolites profiling of protein enriched oyster mushroom (Pleurotus ostreatus (Jacq.) P. Kumm.) grown on oil palm empty fruit bunch substrate. LMT-Food Science and Technology, 181, 1-7.
Inyod, T., Chawananorasest, K., Termarom, T., Konee, C., Tatsom, S., Bualoi, S., et al. (2021a). Quantity and quality yields of oyster mushroom: Pleurotus Pulmonorius TISTR_Ppul-07 and Pleurotus ostreatus TISTR_Post-01 with waste coffee grounds. Khon Kaen Agriculture Journal, 49(6), 1551-1562. (in Thai).
Inyod, T., Termarom, T., Konee, C., Yatsom, S., Bualoi, S. & Eamprasong, P. (2021b). Suitable conditions for production of beta-grucan in oyster mushroom fruiting body. Journal of Science and Technology Mahasarakham University, 40(4), 352-363. (in Thai).
Kumar, S., Kumar, A., Chand, G., Akhtar, M. N. & Kumar, T. (2018). Optimization of growth parameters for Pleurotus florida and Pleurotus sajor-caju. International Journal of Current Microbiology and Applied Sciences, 7, 44814-4823.
Maswko, K. H., Regnier, T., Wokadala, O. C., Bartels, P. & Meiring, B. (2024). Effect of culture media on yield and protein content of Pleurotus ostreatus (Jacq.) Kumm mycelia. International Journal of Food Science, 1,1-14.
Rana, K., Shyaula, M., Bade, A. & Raut, J. K. (2024). Development of improved strains of Pleurotus ostreatus with a shorter harvesting period and a higher yield through hybridization. Asian Journal of Mycology, 7(1), 78-85.
Rungjindamai, N., Trakunjarunkit, K., Posalee, T. & Limpanya, D. (2024). Utilization of agriculture waste for the cultivation of Pleurotus mushrooms in Thailand. Journal of pure and applied microbiology, 18(2), 941-950.
Seeprasong, C., Surawut, S. & Nak-eiam. (2023). Investigation of using agarwood sawdust on growth and yield of the Bhutan oyster mushroom cultivated. Journal of Science and Science Education, 6(1), 31-39. (in Thai).
Subedi, S., Kunwar, N., Pandey, K. R. & Joshi, Y. R. (2023). Performance of oyster mushroom (Pleurotus ostreatus) on paddy straw, water hyacinth and their combinations. Heliyon, 9(8), 1-8.
Suwannarach, N., Kumla, J., Zhao, Y. & Kakumyan, P. (2022). Impact of cultivation substrate and microbial community on improving mushroom productivity: A review. Biology, 11(4), 1-27.
Vilas, P. M., Jadhav, A. C., Dhavale, M. C., Hasabnis, S. N., Gaikwad, A. P., Jadhav, P. R. & Ajit, P. S. (2020). Effect of cultural variability on mycelial growth of eleven mushroom isolates of Pleurotus spp. Journal of Pharmacognosy and Phytochemistry, 9(6), 881-888.
Yu, Y., Liu, T., Wang, Y., Liu, L., He, X., Li, J., Martin, F. M., Peng, W. & Tan, H. (2024). Comparative analyses of Pleurotus pulmonarius mitochondrial genomes reveal two major lineages of mini oyster mushroom cultivars. Computational and Structural Biotechnology Journal, 23, 905-917.