Conservation of Indigenous Thai Mango Cultivars by In Vitro Cultures

Abstract

While commercial cultivars of mango are renowned, cultivated area of indigenous cultivars become lower and will affect mango diversity in Thailand. This research aimed to conserve 8 indigenous mango cultivars by in vitro culture using nucellus tissue. Media and condition for browning control were tested and found that MS in the dark is the best. Then media was changed to MS + 0.1 mg/l NAA for a month and transferred into MS + 0.1 mg/l BA in the last 2 months. After experiments, plantlets were produced with the average shoot and root height at 3.14 ± 2.30 and 4.53 ± 2.33 cm in order.