Antibiogram and Heavy Metal Resistance Pattern of Flavobacterium spp. Isolated From Asian Seabass (Lates Calcarifer) Hatchery

Abstract

This paper described antibiogram and heavy metal resistance pattern of Flavobacterium spp. isolated from Asian Seabass (Lates calcarifer) commercial hatchery. Flavobacterium spp. was recognized as a causative agent of bacterial systemic disease in Asian seabass and may cause a huge loss to fish farmer. Therefore, this study was conducted to help fish farmer in selecting suitable antibiotics in controlling this bacterial. In the present study, a total of 150 Flavobacterium spp. was successfully isolated from diseased Asian Seabass fingerling and water sample from the fingerling tank by using cytophaga medium. The sensitivity of the bacteria against 14 antibiotics; oxolinic acid (2 μg), ampicillin (10 μg), erythromycin (15 μg), furazolidone (15 μg), lincomycin (15 μg), colistin sulphate (25 μg), oleandomycin (15 μg), doxycycline (30 μg), fosfomycin (50 μg), nitrofuratoin (50 μg), florfenicol (30 μg), flumequine (30 μg), tetracycline (30 μg) and spiramycin (100 μg) and four heavy metals mercury (Hg2+), cadmium (Cd2+), chromium (Cr6+) and copper (Cu2+) were determined. In the present study, high percentage of antibiotic resistance case (71.4%) was observed compared to the intermediary sensitive (10.5%) and sensitive case (18.1%) among the present bacterial isolates. The occurrence of high percentage of antibiotic resistance case might due to over or misuse of the tested antibiotics in the hatchery.