The potential of cinnamon, Cinnamomum zeylanicum essential oil as a natural ovicide against cotton leafworm, Spodoptera littoralis

Main Article Content

M. Khedr
H. Sabry
R. El–Gendy

Abstract

The present work deals with the extraction of volatile oil from dried bark of Cinnamomum zeylanicum. The volatile oil was extracted by the common hydro distillation method and determined by GC–MS. A total of 12 components were identified. The major component in the essential oil was the aromatic compound E, Z–cinnamaldehyde (31.91%), followed by the main groups, monoterpenoids or oxygenated monoterpenes (27.25%), sesquiterpene hydrocarbons (15.29%) and sesquiterpenoids (10.69%). The essential oil possessed ovicidal activity against three-day old eggs of Spodoptera littoralis (Boisduval) with LC50 and LC90 values of 1.64 and 7.42%, respectively. Dipping eggs in LC50 values might be indicated the presence of abnormalities in the external morphology of eggshell, chorion surface and micropyle area as compared to untreated eggs using a scanning electron microscope. Additionally, the tested oil significantly reduced total soluble protein, total lipids and activities of both acid phosphatase and phenoloxidase enzymes as compared to control. Moreover, the oil affected some biological parameters such as incubation period of the eggs and subsequent offspring, i.e., larval mortality, larval and pupal duration and pupal weight compared to the control. This information might help in developing IPM strategies against this serious pest

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References

Abbott, W.S. 1925. A method for computing the effectiveness of an insecticide. J. Econ. Entomol. 18(2): 265–267.

Al-Fekaiki, D.F., A.K. Niamah and S.T.G. Al–Sahlany. 2017. Extraction and identification of essential oil from Cinnamomum zeylanicum barks and study the antibacterial activity. J. Microbiol., Biotechnol. Food Sci. 7(3): 312–316.

Al–Sahlany, S.T.G. 2016. Effect of Mentha piperita essential oil against Vibrio spp. isolated from local cheeses. Pak. J. Food Sci. 26(2): 65–74.

Aminzare, M., J. Aliakbarlu and H. Tajik. 2015. The effect of Cinnamomum zeylanicum essential oil on chemical characteristics of Lyoner–type sausage during refrigerated storage. Vet. Res. Forum. 6(1): 31–39.

Benelli, G., R. Pavela, F. Maggi, R. Petrelli and M. Nicoletti. 2017. Commentary: making green pesticides greener? The potential of plant products for nanosynthesis and pest control. J. Clus. Sci. 28(1): 3–10.

Bernhard, R.A., T. Shibamoto, K. Yamaguchi and E. White. 1983. The volatile constituents of Schinus molle L. J. Agric. Food Chem. 31(2): 463–466.

Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of proteins utilizing the principle of protein–dye binding. Anal. Biochem. 72: 248–254.

Brattsten, L.B. 1983. Cytochrome P–450 involvement in the interaction between plant terpenes and insect herbivores, pp. 173–195. In: P.A. Hedin, (Ed.), Plant Resistance to Insects. American Chemical Society, Washington, USA.

Dwi Sutanto, K., S. El Salamouny, M. Tufail, R.K.S. Ghulam, M. Sukirno, M.S. Shepard and A.A. Saad. 2017. Evaluation of natural additives to enhance the persistence of Spodoptera littoralis (Lepidoptera: Noctuidae) nucleopolyhedrovirus (SpliMNPV) under field conditions in Saudi Arabia. J. Econ. Entomol. 110: 924–930.

El–Defrawi, M.E., A. Toppozada, N. Mansour and M. Zeid. 1964. Toxicological studies on the Egyptian cotton leafworm, Prodenia litura. I. susceptibility of different larval instars of Prodenia to insecticides. J. Econ. Entomol. 57(4): 591–593.

El–Kordy, M.W., A.I. Gadallah, M.G. Abbas and S.A. Mostafa. 1995. Effect of pyriproxyfen, flufenoxuron and teflubenzuron on some biochemical aspects of Spodoptera littoralis. Al–Azhar J. Agric. Res. 21: 223–238.

EPPO (European and Mediterranean Plant Protection Organization). 1990. Specific Quarantine Requirements. EPPO Technical Documents, No. 1008. European and Mediterranean Plant Protection Organization, Paris, France.

Finney, D.J. 1971. Probit Analysis: A Statistical Treatment of the Sigmoid Response Curve. 3rd edition. Cambridge University Press, Cambridge, UK.

Golmakani, M.T. and K. Rezaei. 2008. Comparison of microwave–assisted hydrodistillation with the traditional hydrodistillation method in the extraction of essential oils from Thymus vulgaris L. Food Chem. 109(4): 925–930.

Ishaaya, I. 1971. Observations on the phenoloxidase system in the armored scales Aonidiella aurantii and Chrysomphalus aonidum. Comp. Biochem. Physiol. B Comp. Biochem. 39(4): 935–943.

Jumbo, L.O.V., K. Haddi, L.R.D. Faroni, F.F. Heleno, F.G. Pinto and E.E. Oliveira. 2018. Toxicity to, oviposition and population growth impairments of Callosobruchus maculatus exposed to clove and cinnamon essential oils. PloS One 13(11): e0207618.

Khedr, M.A. 2016. Ovicidal activity of Sesamum indicum (L.) oil against Spodoptera littoralis (Boisd.). Arc. Phytopathol. Plant Protec. 49(5–6): 95–110.

Khedr, M. and H. El–Kawas. 2013. Control of Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) and Tetranychus urticae Koch (Acari: Tetranychiclae) by coriander essential oil. J. Entomol. 10(4): 170–181.

Kim, S.R., R. Yao, Q. Han, B.M. Christensen and J. Li. 2005. Identification and molecular characterization of a prophenoloxidase involved in Aedes aegypti chorion melanization. Insect Mol. Biol. 14(2): 185–194.

Knight, J.A., S. Anderson and J.M. Rawie. 1972. Chemical basis of the sulfophosphovanilin reaction for estimating total serum lipids. Clin. Chem. 18(3): 199–202.

Kostyukovsky, M., A. Rafaeli, C. Gileadi, N. Demchenko and E. Shaaya. 2002. Activation of octopaminergic receptors by essential oil constituents isolated from aromatic plants: possible mode of action against insect pests. Pest Manag. Sci. 58(11): 1101–1106.

Li, X.Z. and Y.H. Liu. 2007. Diet influences the detoxification enzyme activity of Bactrocera tau (Walker) (Diptera: Tephritidae). Acta Entomol. Sin. 50(10): 989–995.

Li, Z., R. Huang, W. Li, D. Cheng, R. Mao and Z. Zhang. 2017. Addition of cinnamon oil improves toxicity of rotenone to Spodoptera litura (Lepidoptera: Noctuidae) larvae. Flo. Entomol. 100(3): 515–522.

Mahmoud, S.N. 2012. Antifungal activity of Cinnamomum zeylanicum and Eucalyptus microtheca crude extracts against food spoilage fungi. Euphrates J. Agric. Sci. 4: 26–39.

Marcus, C. and E.P. Lichtenstein. 1979. Biologically active components of anise: toxicity and interactions with insecticides in insects. J. Agric. Food Chem. 27(6): 1217–1223.

Mead, H.M., S.N. El–Shafiey and H.M. Sabry. 2016. Chemical constituents and ovicidal effects of mahlab, Prunus mahaleb L. kernels oil on cotton leafworm, Spodoptera littoralis (Boisd.) eggs. J. Plant Protec. Res. 56(3): 279–290.

Moawad, S.S. and H.E. Sadek. 2018. Evaluation of two eco friendly botanical oils on cotton leaf worm, Spodoptera littoralis (Boisd.) (Lepidoptera/Noctuidae). Annal. Agric. Sci. 63(2): 141–144.

Naqqash, M.N., A. Gökçe, A. Bakhsh and M. Salim. 2016. Insecticide resistance and its molecular basis in urban insect pests. Parasitol. Res. 115(4): 1363–1373.

OEPP/EPPO. 2015. EPPO Standards PM 7/124(1) Diagnostic Protocol for Spodoptera littoralis, Spodoptera litura, Spodoptera frugiperda, Spodoptera eridania. Bull. OEPP/ EPPO Bull. 34: 257–270.

Pavela, R. 2010. Acute and synergistic effects of monoterpenoid essential oil compounds on the larvae of Spodoptera littoralis. J. Biopestic. 3(3): 573–578.

Pavela, R. 2016. History, presence and perspective of using plant extracts as commercial botanical insecticides and farm products for protection against insects-a review. Plant Protec. Sci. 52(4): 229–241.

Plata–Rueda, A., J.M. Campos, G. da Silva Rolim, L.C. Martínez, M.H. Dos Santos, L. Fernandes, J.E. Serrão and J.C. Zanuncio. 2018. Terpenoid constituents of cinnamon and clove essential oils cause toxic effects and behavior repellency response on granary weevil, Sitophilus granarius. Ecotoxicol. Environ. Safe. 156: 263–270.

Powell, M.E.A. and M.J.H. Smith. 1954. The determination of serum acid and alkaline phosphatase activity with 4–aminoantipyrine (AAP). J. Clin. Pathol. 7(3): 245–248.

Snedecor, G.W. and W.G. Cochran. 1980. Statistical Methods. 7th edition. Iowa State University Press, Ames, Iowa, USA.

Tripathi, A.K., S. Upadhyay, M. Bhuiyan and P.R. Bhattachary. 2009. A review on materials and methods prospects of essential oils as biopesticide in insect–pest management. J. Pharmacog. Phytother. 1(5): 52–63.

Tsao, R. and J.R. Coats. 1995. Starting from nature to make better insecticides. Chemtech. 25(7): 23–28.

Tsumuki, H. and K. Kanehisa. 1984. Phosphatases in the rice stem borer, Chilo supperssalis Walker (Lepidoptera: Pyralidae): some properties and changes of the activities during hibernation. Cryobiology 21(2): 177–182.

Ziegler, R. and R. Van Antwerpen. 2006. Lipid uptake by insect oocytes. Insect Biochem. Mol. Biol. 36(4): 264–272.