A simple and ecologically friendly method for Jatropha curcas tissue culture

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P. Boonyanan
M. Ketudat-Cairns

Abstract

Some chemicals used in plant tissue culture are toxic and may cause pollution. They are not environmentally friendly and can cause damage to plant tissue, depending on the species and explant used. Therefore, this research aimed to find the most efficient and non-hazardous disinfectant used for Jatropha curcas leaves tissue culture. Optimum hormone balance for J. curcas callus culture as well as for indirect organogenesis was also tested. When 5% hydrogen peroxide (H2O2) was used as a disinfectant, 47.0% green explants without contamination were observed. Callus was generated with the combination of 6-benzyladenine (BA) and indole-3-butyric acid (IBA). The J. curcas shoots were inducted on Murashige and Skoog (MS) medium supplemented with 2 mg L–1 BA. These conditions provided 43.7% regeneration and an average of 20 shoots per 10 calli with an average shoot length of 17.8 mm. Roots were generated by transferring shoots to a root induction medium containing halfstrength MS medium supplemented with 0.5 mg L–1 IBA and 100 mg L–1 phloroglucinol. A protocol of ecologically friendly disinfection and media for J. curcas tissue culture were successfully developed.

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References

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