A simple and ecologically friendly method for Jatropha curcas tissue culture

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P. Boonyanan
M. Ketudat-Cairns


Some chemicals used in plant tissue culture are toxic and may cause pollution. They are not environmentally friendly and can cause damage to plant tissue, depending on the species and explant used. Therefore, this research aimed to find the most efficient and non-hazardous disinfectant used for Jatropha curcas leaves tissue culture. Optimum hormone balance for J. curcas callus culture as well as for indirect organogenesis was also tested. When 5% hydrogen peroxide (H2O2) was used as a disinfectant, 47.0% green explants without contamination were observed. Callus was generated with the combination of 6-benzyladenine (BA) and indole-3-butyric acid (IBA). The J. curcas shoots were inducted on Murashige and Skoog (MS) medium supplemented with 2 mg L–1 BA. These conditions provided 43.7% regeneration and an average of 20 shoots per 10 calli with an average shoot length of 17.8 mm. Roots were generated by transferring shoots to a root induction medium containing halfstrength MS medium supplemented with 0.5 mg L–1 IBA and 100 mg L–1 phloroglucinol. A protocol of ecologically friendly disinfection and media for J. curcas tissue culture were successfully developed.


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Akbar, E., Z. Yaakob, S.K. Kamarudin, M. Ismail and J. Salimon. 2009. Characteristic and composition of Jatropha curcas oil seed from Malaysia and its potential as biodiesel feedstock feedstock. Eur. J. Sci. Res. 29: 396–403.

Al Ghasheem, N., F. Stanica, A.G. Peticila and O. Venat. 2018. In vitro effect of various sterilization techniques on peach (Prunus persica (L.) Batsch) explants. Sci. Papers Ser. B Hortic. 62: 227–234.

Balaji, R., N. Rekha, M. Deecaraman and L. Manik. 2009. Antimetastatic and antiproliferative activity of methanolic fraction of Jatropha curcas against B16F10 melanoma induced lung metastasis in C57BL/6 mice. Afr. J. Pharm. Pharmacol. 3(11): 547–555.

Cao, X. and F.A. Hammerschlag. 2000. Improved shoot organogenesis from leaf explants of highbush blueberry. HortScience. 35: 945–947.

Da Silva, J.A.T., B. Winarto, J. Dobránszki and S. Zeng. 2015. Disinfection procedures for in vitro propagation of Anthurium. Folia Hortic. 27: 3–14.

Datta, M.M., P. Mukherjee, B. Ghosh and T.B. Jha. 2007. In vitro clonal propagation of biodiesel plant (Jatropha curcas L.). Curr. Sci. 93: 1438–1442.

Daud, N., A. Faizal and D. Geelen. 2013. Adventitious rooting of Jatropha curcas L. is stimulated by phloroglucinol and by red LED light. In Vitro Cell. Dev. Biol. Plant. 49: 183–190.

Dos Santos Costa, D., C.C.A. Da Silva, A.J.R. Da Silva, N. Albarello, I.C.N. Direito and C.P. Victorio. 2020. Residual 2,4-D in plant tissue culture discarded media: a neglected source of environmental pollution. Braz. J. Dev. 6: 30046–30059.

Guma, T.B., K. Jane, O. Justus and P.N. Kariuki. 2015. Standardization of in vitro sterilization and callus induction protocol for leaf explants of Anchote: Coccinia abyssinica. Int. J. Res. Dev. Pharm. Life Sci. 4: 1427–1433.

Khurana-Kaul, V., S. Kachhwaha and S.L. Kothari. 2010. Direct shoot regeneration from leaf explants of Jatropha curcas in response to thidiazuron and high copper contents in the medium. Biol. Plant. 54: 369–372.

Lazo-Javalera, M.F., R. Troncoso-Rojas, M.E. Tiznado-Hernández, M.A. Martínez-Tellez, I. Vargas-Arispuro, M.A. Islas-Osuna and M. Rivera-Domínguez. 2016. Surface disinfection procedure and in vitro regeneration of grapevine (Vitis vinifera L.) axillary buds. Springerplus. 5: 453–461.

Li, Z.G., M. Gong, S.Z. Yang and W.B. Long. 2012. Efficient callus induction and indirect plant regeneration from various tissues of Jatropha curcas. Afr. J. Biotechnol. 11(31): 7843–7849.

Liu, Y., J. Lu, H. Zhu, L. Li, Y. Shi and X. Yin. 2016. Efficient culture protocol for plant regeneration from cotyledonary petiole explants of Jatropha curcas L. Biotechnol. Biotechnol. Equip. 30(5): 907–914.

Liu, Y., X. Tong, W. Hui, T. Liu, X. Chen, J. Li and Z. Liu. 2015. Efficient culture protocol for plant regeneration from petiole explants of physiologically mature trees of Jatropha curcas L. Biotechnol. Biotechnol. Equip. 29(3): 479–488.

Mazumdar, P., A. Basu, A. Paul, C. Mahanta and L. Sahoo. 2010. Age and orientation of the cotyledonary leaf explants determine the efficiency of de novo plant regeneration and Agrobacterium tumefaciens–mediated transformation in Jatropha curcas L. S. Afr. J. Bot. 76(2): 337–344.

McDonnell, G. and A.D. Russell. 1999. Antiseptics and disinfectants: activity, action, and resistance. Clin. Microbiol. Rev. 12(1): 147–179.

Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15: 473–497.

Mweu, C.M., A. Nyende and J. Onguso. 2016. Efficient somatic embryogenesis of Jatropha curcas L. from petiole and leaf discs. Int. J. Biotechnol. Mol. Biol. Res. 7: 29–35.

Nguyen, T.T., T.D. Nguyen, X.T. Dao, T.D. Chu and X.B. Ngo. 2018. In vitro propagation of a Vietnam endemic lady’s slipper orchid (Paphiopedilum vietnamense O. Gruss & Perner). J. Hortic. Plant Res. 1: 1–8.

Paranjape, K., V. Gowariker, V.N. Krishnamurthy and S. Gowariker. 2014. The Pesticide Encyclopedia. CABI, Oxfordshire, UK.

Parawira, W. 2010. Biodiesel production from Jatropha curcas: a review. Sci. Res. Essays. 5: 1796–1808.

Schaller, G.E., I.H. Street and J.J. Kieber. 2014. Cytokinin and the cell cycle. Curr. Opin. Plant Biol. 21: 7–15.

Shukla, P., V. Makwana, D. Bhatt and P. Robin. 2013. Efficient method for direct and indirect organogenesis in biofuel crop Jatropha curcas. Int. J. Pharm. Bio. Sci. 4(1): 673–682.

Subroto, A.P., C. Utomo, C. Darmawan, R. Hendroko and T. Liwang. 2014. Tissue culture media optimization and genetic transformation of Jatropha curcas genotype Jatromas cotyledon explants. Energy Procedia. 47: 15–20.

Tsegay, E.Z., S. Premjet, A. Kongbangkerd and D. Premjet. 2017. Direct shoot organogenesis from juvenile cotyledon of Jatropha podagrica Hook. Asian J. Plant Sci. Res. 7(2): 17–22.

Yadav, A.K., Y. Prasad, S. Prakash, P. Chand, B.S.G. Singh and G. Singh. 2017. Effects of surface sterilization agents on in vitro plant growth in banana cultivar “Grand Naine”. Int. J. Chem. Stud. 5(4): 1744–1747.

Zhao, L., Y. Diao, S. Jin, F. Zhou and Z. Hu. 2016. Effect of genotype and inflorescence developmental stage on callus induction and plant regeneration of Miscanthus lutarioripariu, a non-food biomass crop. Pak. J. Bot. 48(2): 717–722.