Genetic and heritability studies of grain yield and other agronomic traits in low-N maize

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G.O. Agbowuro
A.E. Salami


A research was conducted at the Biological Garden of Elizade University, Ilara-Mokin, Nigeria to investigate gene actions and heritability estimates for grain yield and other agronomic traits in low-N maize using North Carolina Mating Design III. Two maize inbred lines were crossed to get F1 and proceed to F2. Four randomly selected F2 segregants that serve as male were backcrossed with each of the two parents inbred lines (P1 and P2) that serve as female. The crosses generated were evaluated using a randomized complete block design with three replications in the late cropping seasons in 2019 and early cropping season in 2020 under two environments (low and high nitrogen conditions). General combining ability of females was significant for all study traits at P < 0.05 while general combining ability of males was significant for all study traits at different probability levels. Specific combining ability was significant for the traits studied (P < 0.05) except leaf blight. The environment was also significant for all traits at P < 0.05 excepted ear rot that was significant at P < 0.01. There was a preponderance of dominance genetic variance for ear height, days to 50% silking, and grain yield while additive genetic variance prevailed over other traits. However, the average dominance ratio was lesser than unity in most of the traits studied. The narrow-sense heritability estimates for study traits ranged from 1.25–79.08%. The results revealed that the additive and dominant gene actions were both important for agronomic traits in low-N maize. Hence, the adoption of reciprocal recurrent selection becomes necessary in incorporating low-N traits into selected elite maize cultivars.


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Acquaah, G. 2012. Principles of Plant Genetics and Breeding. 2nd Edition. Wiley-Blackwell, Oxford, UK.

Adeosun, P.K., J.E. Ihemezie, O.C. Ume and U.L. Egu. 2019. The nexus between maize importation, local production and local prices: empirical analysis from Nigeria. Alanya Academic Review. 3(2): 201–213.

Afolabi, M.S., A.E. Salami and G.O. Agbowuro. 2019. Genetic studies of grain yield and other agronomic traits of low-n maize (Zea mays L.) using a diallel cross under nitrogen fertilizer levels. IJPBCS. 6(3): 569–574.

Agboola, A.A. and R.B. Corey. 1973. The relationship between soil pH, organic matter, available P, exchangeable K, Ca, Mg and nine elements in maize tissue. Soil Sci. 115: 367–375.

Agbowuro, G.O. and A.E. Salami. 2015. Performance of low-N maize hybrids (Zea mays L.) and relationship among traits under varied soil nitrogen conditions. J. Res. Agric. Sci. 3(1–2): 52–56.

Ansari, B.A., K.A. Ansari and A. Khund. 2004. Extent of heterosis and heritability in some quantitative characters of bread wheat. Indus. J. Plant Sci. 3: 189–192.

Aune, J.B. and R. Lal. 1997. Agricultural productivity in the tropics and critical limits of properties of Oxisols, Ultisols and Alfisols. Trop. Agric. 74(2): 96–103.

Badu-Apraku, B. 2010. Effects of recurrent selection for grain yield and Striga resistance in an extra-early maize population. Crop Sci. 50: 1735–1743.

Badu-Apraku, B., R.O. Akinwale, M.A.B. Fakorede, M. Oyekunle and J. Franco. 2012. Relative changes in genetic variability and correlations in an early-maturing maize population during recurrent selection. Theor. Appl. Genet. 125: 1289–1301.

Bello, O.B. and G. Olaoye. 2009. Combining ability for maize grain and other agronomic characters in the tropical southern savanna ecology of Nigeria. Afr. J. Biotechnol. 8(11): 2518–2522.

Below, F.E., P.S. Bradua, R.J. Lambert and R.H. Teyker RH. 1997. Combining ability for nitrogen use in maize, pp. 316–319. In: G.O. Edmeades, M. Bänziger, H.R. Mickelson and C.B. Peña-Valdivia, (Eds.), Developing Drought and Low-N Tolerant Maize. Proceedings of a Symposium. CIMMYT, El Batan, Mexico.

Betran, F.J., D. Beck, M. Bäanziger and G.O. Edmeades 2013. Genetic analysis of inbred and hybrid grain yield under stress and non-stress environments in tropical maize. Crop Sci. 43: 807–817.

Burton, G.W. 1952. Quantitative inheritance in grasses, pp. 277–283. In: Proceeding of the 6th International Grass Congress, Volume.1. Pennsylvania State College, Pennsylvania, USA.

Carsky, R.J. and E.N.O. Iwuafor. 1995. Contribution of soil fertility research and maintenance to improved maize production and productivity in Sub-Saharan Africa. In: Proceeding of Regional Maize Workshop. International Institute of Tropical Agriculture, Cotonuo, Benin Republic.

Derera, J., P. Tongoona, B.S. Vivek and M.D. Laing. 2008. Gene action controlling grain yield and secondary traits in southern African maize hybrids under drought and non-drought environments. Euphytica. 162: 411–422.

Elia, F.M., G.L. Hosfield, J.D. Kelly and M.A. Uebersax. 1997. Genetic analysis and interrelationships between traits for cooking time, water absorption, and protein and tannin content of Andean dry beans. J. Amer. Soc. Hort. Sci. 122(4): 512–518.

Falconer, D.S. and T.F.C. Mackay. 1996. Introduction to Quantitative Genetics. 4th Edition. Longman, Harlow, Essex, UK.

Gama, E.E.G., A.R. Hallauer, R.G. Ferrão and D.M. Barbosa. 1995. Heterosis in maize single crosses derived from a yellow Tuxpeño variety in Brazil. Braz. J. Genet. 18: 81–85.

Hallauer, A.R., M.J. Carena and J.B. Miranda. 2010. Quantitative Genetics in Maize Breeding. Springer, Science Business Media, LLC, New York, USA.

Jaliya, M.M., A.M. Falaki, M. Mahmud, Y.A. Sani. 2008. Effect of sowing date and NPK fertilizer rate on yield and yield components of quality protein maize (Zea mays L.). ARPN J. Agric. Biol. Sci. 3(2): 23–29.

Jat, M.L., T. Satyanarayana, K. Manundar, C.M. Parihar, S.L. Jat, J.P. Tetarwal, R.K. Jat and Y.S. Saharawat. 2013. Fertilizer best management practices for maize systems. Indian J. Fert. 9(4): 80–94.

Kant, S., Y.M. Bi and S.J. Rothstein. 2011. Understanding plant response to nitrogen limitation for the improvement of crop nitrogen use efficiency. J. Exp. Bot. 62: 1499–1509.

Kasantonis, N., A. Gagianas and N. Fotiadis. 1988. Genetic control of nitrogen uptake, reduction and partitioning in maize (Zea mays L.). Maydica. 33: 99–108.

Keimeso, Z. and D. Abakemal. 2020. Combining ability of highland adapted maize (Zea Mays. L) inbred lines for desirable agronomic traits under optimum and low nitrogen conditions. J. Sci. Sustain. Dev. 8(1): 1–13.

Li, P., F. Chen, H. Cai, J. Liu, Q. Pan, Z. Liu, R. Gu, G. Mi, F. Zhang and L. Yuan. 2015. A genetic relationship between nitrogen use efficiency and seedling root traits in maize as revealed by QTL analysis. J. Exp. Bot. 66(11): 3175–3188.

Mahalingam, A., S. Robin, K. Mohanasundaram and R. Pushpam. 2011. Studies on genetic architecture of biparental progenies for important yield attributes in rice (Oryza sativa L.). J. Plant Breed. Crop Sci. 3(12): 296–301.

Martins, A.O., E. Campostrini, P.C. Magalhães, L.J.M. Guimarães, F. Ozanan, M. Durães, I.E. Marriel and A.T. Netto. 2008. Nitrogen-use efficiency of maize genotypes in contrasting environments. Crop Breed. Appl. Biotechnol. 8: 291–298.

Ogunboye, O.I., A.O. Adekiya, B.S. Ewulo and O.A. Adeniyi. 2020. Effects of split application of urea fertilizer on soil chemical properties, maize performance and profitability in southwest Nigeria. Open Agric. J. 14: 36–42.

Oikeh, S.O. 1996. Dynamics of Soil Nitrogen in Cereal-Based Cropping Systems in the Nigerian Savanna. PhD Thesis, Ahmadu Bello University, Zaria, Nigeria.

Rizzi, E., C. Balconi, L. Nembrini, F.M. Stefanini, F. Coppolino and M. Motto. 1993. Genetic-variation and relationships among N-related traits in maize. Maydica. 38(1): 23–30.

Robinson, H.F., R.E. Comstock and P.H. Harvey. 1955. Estimates of heritability and the degree of dominance in maize. Agron. J. 41: 353–359.

Salami, A.E. and G.O. Agbowuro. 2016. Gene action and heritability estimates of grain yield and disease incidence traits of low-N maize (Zea mays L.) inbred lines. Agriculture and Biology Journal of North America. 7(2): 50–54.

Sardana, S., R.K. Mahajan, N.K. Gautam and B. Ram. 2007. Genetic variability in pea (Pisum sativum L.) germplasm for utilization. J. Breed. Genet. 39(1): 31–41.

SAS. 1995. SAS/STAT User’s Guide: Version 6, Vol. I and II. 4th Edition. SAS Institute Inc., Cary, North Carolina, USA.

Sharma, J.R. 2006. Statistical and Biometrical Techniques in Plant Breeding. 1st Edition. New Age International (P) Limited, Publishers, New Delhi, India.

Sobulo, R.A. and A.O. Osiname. 1981. Soils and fertilizer use in the western in Nigeria. Research Bulletin No.11. Institute of Agricultural Research and Training, University of Awolowo, Ile-Ife, Nigeria.

Sunday, I., A. Babatunde, A. Stephen, A. Charity and O. Kayode. 2020. Gene action in low nitrogen tolerance and implication on maize grain yield and associated traits of some tropical maize populations. Open Agric. 5: 801–805.

Tilman, D., K.G. Cassman, P.A. Matson, R. Naylor and S. Polasky. 2002. Agricultural sustainability and intensive production practices. Nature. 418: 671–677.

Wolfe, D.W., D.W. Henderson, T.C. Hsiao and A. Alvino. 1988. Interactive water and nitrogen effects on maize. II. Photosynthetic decline and longevity of individual leaves. Agron. J. 80: 865–870.