Efficacy of Carica papaya, Areca catechu, and Manihot esculenta extracts against Ascaridia galli eggs (In vitro)
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Abstract
Background and Objective: The anthelmintic efficacy of three plant-based extracts, such as papaya seeds, betel nut fruit, and cassava leaves was evaluated against the larval development of Ascaridia galli eggs, in vitro.
Methodology: A total of 2,000–2,500 A. galli eggs isolated from the intestines of native chickens were randomly distributed into 5 treatments following the complete randomized design (CRD). Treatments were: T1: piperazine, T2: phosphate-buffered saline (PBS), T3: Carica papaya seed extract, T4: Areca catechu extract, and T5: Manihut esculenta leaf extract. Plant-based extracts were diluted in PBS to create a dose concentration of 50 mg/mL.
Main Results: After 21 days of incubation, results revealed that extracts of C. papaya seed, A. catechu, and M. esculenta leaf were as effective as piperazine in inhibiting the development of A. galli eggs during the 7th day of incubation. A. catechu extract showed the highest efficacy over time, ranging from 90–100%, followed by M. esculenta leaf extract at 71–100%, and C. papaya seed extract at 51–100%. Whereas piperazine obtained 100% efficacy in controlling A. galli eggs throughout the experimental trial.
Conclusions: This study indicated that the extracts from C. papaya seed, A. catechu, and M. esculenta leaf could be utilized effectively in controlling the larval development of A. galli eggs as an alternative to piperazine.
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