An implication of apple cider vinegar administration on the hematology, serum biochemistry, and liver histology of broiler chickens

Main Article Content

M.O. Abatan
O.O. Adeleye
A.J. Akamo
M.O. Abioja
L.T. Egbeyale
O.A. Dosumu

Abstract

Background and Objectives: Organic acids (OA) have been discovered to possess antimicrobial properties, thus resulting in growth improvement in animals. Apple cider vinegar (ACV) contains a blend of OAs in addition to its vitamins and minerals constituent. The aim of this study was to evaluate the effect of intermittent usage of ACV as an alternative growth promoter on the hematology, serum biochemistry, and liver histology of broiler chickens.
Methodology: In a 6-week study, 300 Arbor Acres broiler chicks were randomly distributed into 6 treatments of 50 birds, which were replicated 5 times to contain 10 birds per replicate; this was arranged in a 2 × 3 factorial layouts using a completely randomized design. Birds in 4 treatments were offered graded doses of ACV in their water (5 or 10 mL/L) for either 2 or 3 consecutive days/week, while the birds in the 2 control treatments were administered antibiotics (enrofloxacin).
Main Results: All hematological parameters measured were not significant except the heterophil, which increased with increased dosages of ACV. From the interactive effect of dosage and frequency of administration of ACV, the highest and lowest values of heterophil were obtained from birds offered 10 mL/L ACV thrice weekly and 5 mL/L ACV thrice weekly, respectively. Among the serum parameters, aspartate aminotransferase significantly reduced with a higher administration frequency of ACV, while alanine aminotransferase (ALT) was higher in birds given ACV twice (30.70 ± 3.83 U/L) and thrice weekly (31.15 ± 3.83 U/L) compared to birds on antibiotics (15.10 ± 3.85 U/L). Also, the interactive effect of dosage and frequency of administration of ACV showed that birds offered 10 mL/L ACV twice weekly (37.40 ± 5.41 U/L), and one of the groups offered antibiotics (14.60 ± 5.41 U/L) had the highest and lowest ALT values, respectively. Creatinine level was not significant across all treatments. Results of the liver histology revealed that birds offered 10 mL/L ACV had slight necrosis of the hepatocytes and liver parenchyma.
Conclusions: This study discovered that ACV, an organic growth promoter, should be used at a lower dose and in discontinuous frequency for broiler chickens to prevent possible negative impacts on their liver.

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Research Articles

References

Allahdo, P., J. Ghodraty, H. Zarghi, Z. Saadatfar, H. Kermanshahi and M.R.E. Dovom. 2018. Effect of probiotic and vinegar on growth performance, meat yields, immune responses, and small intestine morphology of broiler chickens. Ital. J. Anim. Sci. 17(3): 675–685. https://doi.org/10.1080/1828051X.2018.1424570.

Altmann, R.B. 1979. Avian clinical pathology, radiology, parasitic and infectious diseases. In: Proceedings of the American Animal Hospital Association. South Bend, Indiana, USA.

Avwioro, O.G. 2010. Histochemistry and Tissue Pathology, Principle and Techniques. Claverianum Press, Ibadan, Nigeria.

Bounous, D. and N. Stedman. 2000. Normal avian hematology: chicken and turkey, pp. 1147–1154. In: B.F. Feldman, J.G. Zinkl and N.C. Jain, (Eds), Schalm’s Veterinary Hematology. Wiley, New York, USA.

Budak, N.H., D. Kumbul Doguc, C.M. Savas, A.C. Seydim, T. Kok Tas, M.I. Ciris and Z.B. Guzel-Seydim. 2011. Effects of apple cider vinegars produced with different techniques on blood lipids in highcholesterol-fed rats. J. Agric. Food Chem. 59(12): 6638–6644. https://doi.org/10.1021/jf104912h.

Dacie, J.V. and S.M. Lewis. 1991. Practical Haematology. 7th Edition. Church Hill Livingstone, Edinburgh, England.

Ferket, P.R., A.A. Santos Jr. and E.O. Oviedo-Rondon. 2005. Dietary factors that affect gut health and pathogen colonization, pp. 22. In: Proceedings of the 32nd Annual Carolina Poultry Nutrition Conference. North Carolina, USA.

Fouladi, P., Y. Ebrahimnezhad, H. Aghdam Shahryar, N. Maheri and A. Ahmadzadeh. 2018. Effects of organic acids supplement on performance, egg traits, blood serum biochemical parameters and gut microflora in female Japanese quail (Coturnix coturnix japonica). Rev. Bras. Ciênc. Avic. 20(1): 133–144. https://doi.org/10.1590/1806-9061-2016-0375.

FUNAAB (Federal University of Agriculture, Abeokuta). 2016. Policy on Research of the Federal University of Agriculture, Abeokuta, Nigeria. Available Source: http://www.funaab.edu.ng. March 22, 2021.

Harmon, B.G. 1998. Avian heterophils in inflammation and disease resistance. Poult. Sci. 77(7): 972–977. https://doi.org/10.1093/ps/77.7.972.

Ismail, I.E., M. Alagawany, A.E. Taha, N. Puvača, V. Laudadio and V. Tufarelli. 2021. Effect of dietary supplementation of garlic powder and phenyl acetic acid on productive performance, blood haematology, immunity and antioxidant status of broiler chickens. Anim. Biosci. 34(3): 363–370. https://doi.org/10.5713%2Fajas.20.0140.

Jahantigh, M., H. Kalantari, S.A. Davari and D. Saadati. 2021. Effects of dietary vinegar on performance, immune response and small intestine histomorphology in 1- to 28-day broiler chickens. Vet. Med. Sci. 7(3): 766–772. https://doi.org/10.1002/vms3.408.

Kalaba, V., Z. Marjanović Balaban and D. Kalaba. 2019. Antibacterial activity of domestic apple cider vinegar. AGROFOR International Journal. 4(1): 24–31. https://doi.org/10.7251/AGRENG1901024K.

Kasarala, G. and H.L. Tillmann. 2016. Standard liver tests. Clin. Liver Dis. 8(1): 13–18. https://doi.org/10.1002%2Fcld.562.

Khosravi, A., F. Boldaji, B. Dastar and S. Hasani. 2010. Immune response and performance of broiler chicks fed protexin and propionic acid. Int. J. Poult. Sci. 9(2): 188–191. https://doi.org/10.3923/ijps.2010.188.191.

Kopecký, J., C. Hrnčár and J. Weis. 2012. Effect of organic acids supplement on performance of broiler chickens. Scientific Papers: Animal Sciences and Biotechnologies. 45(1): 51–54.

Lilly, K.G.S., L.K. Shires, B.N. West, K.R. Beaman, S.A. Loop, P.J. Turk, G.K. Bissonnette and J.S. Moritz. 2011. Strategies to improve performance and reduce preslaughter Salmonella in organic broilers. J. Appl. Poult. Res. 20(3): 313–321. https://doi.org/10.3382/japr.2010-00245.

Meluzzi, A., G. Primiceri, R. Giordani and G. Fabris. 1992. Determination of blood constituents reference values in broilers. Poult. Sci. 71(2): 337–345. https://doi.org/10.3382/ps.0710337.

MINITAB. 2018. Minitab Incorporation, State College, Pennsylvania, USA.

Nagatomi, R. 2006. The implication of alterations in leukocyte subset counts on immune function. Exerc. Immunol. Rev. 12: 54–71.

Natera, R., R. Castro, M.J. Hernández and C. García-Barroso. 2003. Chemometric studies of vinegars from different raw materials and processes of production. J. Agric. Food Chem. 51(11): 3345–3351. https://doi.org/10.1021/jf021180u.

NCCLS (National Committee for Clinical Laboratory Standards). 1990. Procedures for the Handling and Processing of Blood Specimens, Approved Guideline. NCCLS Publication H18-A. Villanova, Pennsylvania, USA.

Nosrati, M., F. Javandel, L.M. Camacho, A. Khusro, M. Cipriano, A. Seidavi and A.Z.M. Salem. 2017. The effects of antibiotic, probiotic, organic acid, vitamin C, and Echinacea purpurea extract on performance, carcass characteristics, blood chemistry, microbiota, and immunity of broiler chickens. J. Appl. Poult. Res. 26(2): 295–306. https://doi.org/10.3382/japr/pfw073.

Nourmohammadi, R., S.M. Hosseini and H. Farhangfar. 2010. Effect of dietary acidification on some blood parameters and weekly performance of broiler chickens. J. Anim. Vet. Adv. 9(24): 3092–3097. https://doi.org/10.3923/javaa.2010.3092.3097.

Okubo, S., M. Miyamoto, K. Takami, M. Kanki, A. Ono, N. Nakatsu, H. Yamada, Y. Ohno and T. Urushidani. 2013. Identification of novel liver-specific mRNAs in plasma for biomarkers of drug-induced liver injury and quantitative evaluation in rats treated with various hepatotoxic compounds. Toxicol. Sci. 132(1): 21–31. https://doi.org/10.1093/toxsci/kfs340.

Olafedehan, C.O., A.M. Obun, M.K. Yusuf, O.O. Adewumi, A.O. Oladefedehan, A.O. Awofolaji and A.A. Adeniji. 2010. Effects of residual cyanide in processed cassava peal meals on haematological and biochemical indices of growing rabbits, pp. 212. In: Proceedings of the 35th Annual Conference of Nigerian Society for Animal Production. Ibadan, Nigeria.

Raval, N., D. Kalyane, R. Maheshwari and R.K. Tekade. 2019. Surface modification of biomaterials and their implication on biocompatibility, pp. 639–674. In: R.K. Tekade, (Ed), Advances in Pharmaceutical Product Development and Research: Biomaterials and Bio-nanotechnology. Academic Press.

Reece, W.O. and M.J. Swenson. 2004. The composition and function ofblood, pp. 26–52. In: W.O. Reece, (Ed), Dukes’ Physiology of Domestic Animals. 12th Edition. Comstock Publishing Associates, Ithaca, New York, USA.

Reitman, S. and S. Frankel. 1957. A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am. J. Clin. Pathol. 28(1): 56–63. https://doi.org/10.1093/ajcp/28.1.56.

Rocha, T.M., M.A. Andrade, E. Gonzales, J.H. Stringhini, E.S. Santana, R.N.G. Pôrto and C.S. Minafra-Rezende. 2013. Liver function and bacteriology of organs in broiler inoculated with nalidixic acid-resistant Salmonella Typhimurium and treated with organic acids. Ital. J. Anim. Sci. 12(3): e55. https://doi.org/10.4081/ijas.2013.e55.

Sanchez, O., A. Arnau, M. Pareja, E. Poch, I. Ramirez and M. Soley. 2002. Acute stress-induced tissue injury in mice: differences between emotional and social stress. Cell Stress Chaperones. 7(1): 36–46.

Sikandar, A. 2018. Histopathology: an old yet important technique in modern science, pp. 3–10. In: S. Srivastava, (Ed), Histopathology-An Update. IntechOpen. http://doi.org/10.5772/intechopen.69367.

Swaggerty, C.L., R.J. Arsenault, C. Johnson, A. Piva and E. Grilli. 2020. Dietary supplementation with a microencapsulated blend of organic acids and botanicals alters the kinome in the ileum and jejunum of Gallus gallus. PLoS ONE. 15(7): e0236950. https://doi.org/10.1371/journal.pone.0236950.

Thompson, J.L. and M. Hinton. 1997. Antibacterial activity of formic and propionic acids in the diet of hens and Salmonellas in the crop. Br. Poult. Sci. 38(1): 59–65. https://doi.org/10.1080/00071669708417941.

Togun, V.A., B.S.A. Oseni, J.A. Ogundipe, T.R. Arewa, A.A. Hammed, D.C. Ajonijebu and F. Mustapha. 2007. Effects of chronic lead administration on the haematological parameters of rabbits – a preliminary study, pp. 341. In: Proceedings of the 41st Conferences of the Agricultural Society of Nigeria. Zari, Nigeria.

Ulsagheer Mohanad, K., M.R. Ibrahim Saleem and M.J. Kadhim. 2019. Effect of using natural apple vinegar, garlic powder (Alsin) and black bean seed on the immune system and some of characteristics of the blood broilers Ross 308. J. Phys.: Conf. Ser. 1294(9): 092006. https://doi.org/10.1088/1742-6596/1294/9/092006.

Watkins, S., J. Cornelison, C. Tillery, M. Wilson and R. Hubbard. 2004. Effect of water acidification on broiler performance. Avian Advice. University of Arkansas’s. Available Source: https://www.thepoultrysite.com/articles/effects-of-water-acidification-on-broiler-performance. May 26, 2021.

Zhou, Z., M.J. Xu and B. Gao. 2016. Hepatocytes: a key cell type for innate immunity. Cell. Mol. Immunol. 13: 301–315. https://doi.org/10.1038/cmi.2015.97.