Effect of tris-based extenders with sunflower lecithin or egg yolk on the post-thaw qualities of Kalahari Red buck semen
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Abstract
Background and Objective: This study aimed to determine the effect of sunflower lecithin (SL) concentrations, egg yolk, and sugar types in tris-based extenders on post-thaw qualities of Kalahari Red (KR) buck semen. SL was used as an alternative to egg yolk in the semen extender during cryopreservation due to its characteristic phospholipid content which played a major cryoprotective role and also to prevent the deleterious effect of egg yolk on semen when included in the extender.
Methodology: Semen was collected from four KR bucks between 2 and 2 ½ years of age using an artificial vagina, evaluated for sperm motility, pooled, and then diluted in tris-sucrose and tris-fructose extenders, each containing 1.5, 3.0, 4.5% SL and 20% egg yolk (0% SL). Diluted semen was loaded in 0.25 mL plastic straws, cooled to 4 °C, and suspended in liquid nitrogen (LN) for 10 min. where the temperature reached -120 °C before plunging directly into the LN for 7 days. Sperm qualities, including motility, acrosome integrity, membrane integrity, malondialdehyde (MDA) concentration, seminal arginase activity, and cholesterol level, were evaluated after thawing. Data were analyzed using a 2 × 4 factorial arrangement.
Main Results: Results showed that generally, tris-fructose extender had significantly better results as regards motility, cholesterol level, and seminal arginase activity (23.33 ± 3.25%, 78.62 ± 12.83 mg/dL, and 0.41 ± 0.06 units/mg protein respectively; P < 0.05) while there was no significant difference in acrosome integrity, membrane integrity, and malondialdehyde concentration when compared with tris-sucrose extender. However, interactive results showed that the tris-fructose extender had better (P < 0.05) post-thaw semen qualities with egg yolk. In contrast, the tris-sucrose extender had better (P < 0.05) results with 4.5% (SL) in the cryopreservation of Kalahari buck semen.
Conclusions: Tris-fructose extender with egg yolk had better post-thaw semen qualities than other extenders. However, with a tris-fructose extender where egg yolk is to be replaced with sunflower lecithin, this should be done at 3.0% inclusion, while 4.5% sunflower lecithin inclusion in sucrose extender will also give the desired result.
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