Optimizing culture medium for commercial production of tropical day-blooming waterlily (Nymphaea colorata Peter)
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Abstract
Background and Objective: Tropical day-blooming waterlily, the queen of aquatic ornamental plants, has steadily increased commercial demand. However, the traditional propagation methods result in a low number of plants and lack consistency, which is inefficient in meeting the demands of the global export industry. Therefore, this study aims to develop a protocol for rapid mass propagation of tropical waterlily using tissue culture techniques.
Methodology: Individual aseptic shoots of Nymphaea colorata Peter were cultured on different culture media, including Murashige and Skoog (MS) and Oil Palm Culture Medium (OPCM), with various types and concentrations of plant growth regulators. The experiment was designed as a completely randomized design (CRD) with 20 replications, each replication consisting of 5 shoot tips. After 4 weeks of culture, shoot multiplication rate and number of shoots per explant were recorded. Shoots were transferred to a rooting medium, transplanted in aquatic plant substrates, and transferred directly to a freshwater tank with a 20 cm water depth under greenhouse conditions. The survival rate was recorded.
Main Results: OPCM medium supplemented with 3.0 mg/L BAP and 0.5 mg/L NAA resulted in significantly (P < 0.05) highest growth and shoot proliferation, producing 19.2 shoots per explant. For mass shoot proliferation, culturing shoot explants on OPCM medium supplemented with 0.5 mg/L TDZ in combination with 1.0 mg/L BAP, and 0.5 mg/L NAA showed a significant (P < 0.05) highest response rate of 86.66% and produced the highest number of shoots at 31.15 shoots/explant. Plantlets exhibited robust growth and achieved a high survival rate of 90%, achieving 100%, as evidenced by the production of new floating leaves and shoots under greenhouse conditions.
Conclusions: OPCM medium supplemented with 0.5 mg/L TDZ in combination with 1.0 mg/L BAP, and 0.5 mg/L NAA was suitable for mass propagation of tropical day-blooming waterlily.
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