Examination of persistence of bloodstains on various types of surfaces after thermal exposure by the method of luminol test

Abstract

This research investigated the persistence of bloodstains on diverse surfaces post-thermal exposure using the luminol test, aiming to enhance forensic crime-solving capabilities. Blood, initially deposited in 25 microliters on glass plates, stainless steel, floor tiles, and galvanized sheets, underwent drying for 20 minutes at room temperature. Varied temperature (100°C to 500°C) and exposure times (5, 15, 30, 60 mins) were applied. Results revealed that galvanized sheets exhibited lower luminol detectability compared to glass plates, stainless steel, and floor tiles. Bloodstains on glass plates, stainless steel, and galvanized sheets were identifiable with luminol after exposure to temperatures up to 350°C for 60 minutes, but not at 400°C and 500°C. In contrast, floor tiles maintain detectability across the entire temperature range. This study underscored luminol's efficacy in detecting bloodstains on diverse surfaces post-thermal exposure in authentic forensic scenarios, offering valuable insights for investigators facing varying conditions in criminal cases.