Development of Drugs Sample Preparation in Stomach Content

Abstract

The stomach contents are a typical sample for postmortem analysis of drugs and toxins along with analysis of other biological samples. It was found that in the stomach had background interference from fat interfering with the analysis using the technique GC-MS, thus obscuring the detection of the target drugs. The researcher therefore developed an analysis of drugs and clean-up lipids in stomach content. The purposes were to study the Types of drug extraction solvents, namely Acetonitrile Methanol and Acetonitrile Isopropanol at volumes of 1, 1.5 and 2 mL and to study the types of solvents used for cleaning-up lipids, namely hexane petroleum ether and hexane to petroleum ether mixed solvent at volumes of 1, 2 and 3 mL. Results of the study on drug extraction with Acetonitrile at a volume of 2 mL when used with a Hexane found that Petroleum Ether cleaning-up lipids at a volume of 1 mL.  was able to extract the drug that gave the highest recovery value and the obtained values were tested for differences (pair-t-test). The extraction efficacy of the two drugs was significantly different at the 95% confidence level. When applied to the stomach content samples comparing the cholesterol peak in the sample, the developed method had lower peak height than the traditional method. This method is easy to analyze results, uses a smaller sample volume and can analyze both acidic and basic drugs at one time. It also extends tool life and reduces the cost of analysis