Effect of temperature and storage period of Dendrobium Yaya Victoria pollinia on pollinia viability, pod formation and in vitro growth of seedlings
Keywords:
orchid, pollinia, breeding, seed, protocormAbstract
Storage of pollinia is crucial for hybridization of orchids with different flowering times to produce hybrid orchids. The storage of Dendrobium Yaya Victory pollinia at room temperature (25 °C), 12 °C, and -14 °C for 7-350 d in the forms of fresh pollinia and dried pollinia was investigated to study the optimum temperature and duration for storage of pollinia for pollination. The results showed that the optimum temperature of fresh pollinia storage for 39 d was room temperature, with an average of 80% and 100% of the pollinia viability and pod formation, respectively. An average of 40% of the pollinia viability and pod formation were observed after 128 d of fresh pollinia storage at -14 °C. Seeds of pollination with the stored pollinia produced embryos, and after 40 d of sowing on Vacin & Went (1949) agar medium supplemented with 150 ml/l coconut water and 10 g/l sucrose, the seeds germinated into protocorms. On the modified Vacin & Went (1949) agar medium, which was supplemented with 150 ml/l coconut water, 100 g/l banana, 1 g/l activated charcoal, and 20 g/l sucrose, the protocorms developed into plantlets. Plantlets that were pollinated using stored pollinia were identical to those that were pollinated using fresh pollinia and grew in the greenhouse. For short-term and medium-term pollinia storage for pollination, fresh Dendrobium Yaya Victory pollinia can be stored at room temperature and -14 °C, respectively. This method is easy to use, doesn't require chemicals or costly equipment, and can be used to improve plant breeding in Thai orchids and hybrid orchids.
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