Efficiency of fermented lychee leaf biochar extract for enhancing growth, yield and protection against Mealybug (Phenacoccus longispinus) in yardlong bean

Authors

Keywords:

Biochar, Lychee leaf, Mealybug, Yardlong bean, Organic agriculture

Abstract

This study aimed to evaluate the efficacy of fermented lychee leaf biochar extract on growth, yield, and protection against mealybug (Phenacoccus spp.) in yardlong bean. The experiment was conducted in a greenhouse using a completely randomized design (CRD) to compare the efficacy of four treatments of fermented bio-extract from: lychee biochar, neem, pineapple and tobacco, with four replications each. The bioliquids were sprayed onto yardlong bean plants every three days from 80 to 101 days after planting, for a total of 7 applications. Mealybugs were artificially released onto the inflorescence area at 94 days after transplanting. The results indicated that plant growth among the treatments showed no statistically significant differences; however, the fermented lychee leaf biochar extract tended to promote higher growth compared to other bioliquids. Regarding pest control efficacy, the fermented lychee leaf biochar extract exhibited the highest mean pH value (7.62) and achieved the fastest reduction in mealybug populations (within 7 days). Moreover, it consistently maintained the lowest infestation severity level (scale 1) throughout the experiment and produced the highest pod yield (70.75 g per plant). In conclusion, the fermented lychee leaf biochar extract demonstrated strong potential as a biobased agent for promoting growth and yield of yardlong bean, while effectively controlling mealybug infestation. This product could serve as an alternative to chemical pesticides or other biocontrol agents.

References

Abit, S. M., Bolster, C. H., Cai, P., & Walker, S. L. (2012). Influence of feedstock and pyrolysis temperature of biochar amendments on transport of Escherichia coli in saturated and unsaturated soil. Environmental Science & Technology, 46(15), 8097–8105. https://doi.org/10.1021/es300797z

Arshad, U., Azeem, F., Mustafa, G., Bakhsh, A., Toktay, H., McGliffen, M., Nawaz, M. A., Naveed, M., & Ali, M. A. (2021). Combined application of biochar and biocontrol agents enhances plant growth and activates resistance against Meloidogyne incognita in tomato. Gesunde Pflanzen, 73(2), 591–601. https://doi.org/10.1007/s10343-021-00580-4

Basagli, M. A. B., Moraes, J. C., Carvalho, J. A., Ecole, C. C., & Gonçalves-Gervásio, R. C. R. (2003). Effect of sodium silicate application on the resistance of wheat plants to the green aphids, Schizaphis graminum (Rond.) (Hemiptera: Aphididae). Neotropical Entomology, 32(4), 659–663. https://doi.org/10.1590/S1519-566X2003000400017

Chen, M. S. (2008). Inducible direct plant defense against insect herbivores: A review. Insect Science, 15, 101–114. https://doi.org/10.1111/j.1744-7917.2008.00190.x

Chen, Y., Shen, Y., Li, B., & Meng, L. (2019). The effect of biochar amendment to soils on Cnaphalocrocis medinalis Guenée (Lepidoptera: Pyralidae) on rice. Crop Protection, 124, 104842. https://doi.org/10.1016/j.cropro.2019.104842

Costa, R. R., Moraes, J. C., & Da Costa, R. R. (2011). Feeding behavior of the greenbug Schizaphis graminum on wheat plants treated with imidacloprid and/or silicon. Journal of Applied Entomology, 135(1–2), 115–120. https://doi.org/10.1111/j.1439-0418.2010.01526.x

Currie, H. A., & Perry, C. C. (2007). Silica in plants: Biological, biochemical and chemical studies. Annals of Botany, 100(7), 1383–1389. https://doi.org/10.1093/aob/mcm247

Elad, Y., David, D. R., Harel, Y. M., Borenshtein, M., Kalifa, H. B., Silber, A., & Graber, E. R. (2010). Induction of systemic resistance in plants by biochar, a soil-applied carbon sequestering agent. Phytopathology, 100(9), 913–921. https://doi.org/10.1094/PHYTO-100-9-091

George, A., Broadley, R., Hutton, D., Redpath, S., Bignell, G., Nissen, B., Bruun, D., & Waite, G. (2015). Integrated pest and disease management manual for custard apple. Queensland Department of Agriculture and Fisheries.

Heine, G., Tikum, G., & Horst, W. J. (2007). The effect of silicon on the infection by and spread of Pythium aphanidermatum in single roots of tomato and bitter gourd. Journal of Experimental Botany, 58(3), 569–577. https://doi.org/10.1093/jxb/erl258

Hemwong, S. (2022). Lychee leaf biochar solution for mealybug control. Petty Patent Application No. 223000438. Department of Intellectual Property, Thailand.

Jayappa, B. G., & Lingappa, S. (1988). Screening of cowpea germplasm for resistance to Aphis craccivora Koch in India. Tropical Pest Management, 34(1), 62–64. https://doi.org/10.1080/0967087880937120

Korndörfer, A. P., Grisoto, E., & Vendramim, J. D. (2011). Induction of insect plant resistance to the spittlebug Mahanarva fimbriolata Stål (Hemiptera: Cercopidae) in sugarcane by silicon application. Neotropical Entomology, 40(3), 387–392. https://doi.org/10.1590/S1519-566X2011000300013

Kobrus, A. (1987). Evaluation of yield and quality losses in Turkish tobacco caused by aphids. Journal of Entomology and Zoology, 9(4), 187–193.

Lehmann, J., Rillig, M. C., Thies, J., Masiello, C. A., Hockaday, W. C., & Crowley, D. (2011). Biochar effects on soil biota: A review. Soil Biology & Biochemistry, 43(9), 1812–1836. https://doi.org/10.1016/j.soilbio.2011.04.022

Li, S., Song, Z., Singh, B. P., & Wang, H. (2019). The impact of crop residue biochars on silicon and nutrient cycles in croplands. Science of the Total Environment, 659, 673–680. https://doi.org/10.1016/j.scitotenv.2018.12.381

Lou, Y., Joseph, S., Li, L., Graber, E. R., Liu, X., & Pan, G. (2016). Water extract from straw biochar used for plant growth promotion: An initial test. BioResources, 11(1), 249–266. https://doi.org/10.15376/biores.11.1.249-266

Maruthadurai, R., & Karuppaiah, V. (2014). Managing menace of insect pests on custard apple. Popular Kheti, 2(3), 108–111.

Ng, E. L., & Cavagnaro, T. R. (2016). Biochar effects on ecosystems: Insights from lipid-based analysis. In T. K. Ralebitso-Senior & C. H. Orr (Eds.), Biochar application (pp. 55–77). Elsevier.

Nikpay, A. (2016). Improving biological control of stalk borers in sugarcane by applying silicon as a soil amendment. Journal of Plant Protection Research, 56(4), 394–401. https://doi.org/10.1515/jppr-2016-0058

Rakbankerd. (2024, July 16). Pineapple peel fermented extract for soil improvement and crop yield enhancement. https://www.rakbankerd.com/agriculture/print.php?id=3466&s=tblplant

Raven, J. A. (1983). The transport and function of silicon in plants. Biological Reviews, 58(2), 179–207. https://doi.org/10.1111/j.1469-185X.1983.tb00385.x

Reynolds, O. L., Padula, M. P., Zeng, R., & Gurr, G. M. (2016). Silicon: Potential to promote direct and indirect effects on plant defense against arthropod pests in agriculture. Frontiers in Plant Science, 7, 744. https://doi.org/10.3389/fpls.2016.00744

Teak, K. O. H., Yoshiyuki, S., Jiro, C., Yong, H. L., & Bongsu, C. (2012). Effect of aqueous extract of biochar on germination and seedling growth of lettuce (Lactuca sativa L.). Journal of the Faculty of Agriculture, Kyushu University, 57(1), 55–60. https://doi.org/10.5109/22048

Vasanthi, L. A., Muruganandam, A., Revathi, P., Baskar, B., Jayapriyan, K., Baburajendran, R., & Munuswamy, N. (2014). The application of histo-cytopathological biomarkers in the mud crab Scylla serrata (Forsskål) to assess heavy metal toxicity in Pulicat Lake, Chennai. Marine Pollution Bulletin, 81, 85–93. https://doi.org/10.1016/j.marpolbul.2014.02.017

Wang Sam Mo District Agricultural Office. (2016, July 16). Traditional tobacco extract formula for controlling mealybug and insect pests. http://wangsammo.udonthani.doae.go.th/KM%2009%2059.pdf

Wiriyaudomchi, R., & Kasemsuk, S. (2016). Effect of neem leaf fermented extract on the control of mealybugs in vegetables. Journal of Agriculture Research and Development, 33(2), 45–53.

Published

2026-04-20

How to Cite

Hemwong, S., & Thiphasri, B. (2026). Efficiency of fermented lychee leaf biochar extract for enhancing growth, yield and protection against Mealybug (Phenacoccus longispinus) in yardlong bean. Agriculture & Technology RMUTI Journal, 7(1), 84–95. retrieved from https://li01.tci-thaijo.org/index.php/atj/article/view/267984