Comparison of Different Extraction Methods and Solvent Ratios on Yield, Content of Total Phenolic and Flavonoid in Kaempferia parviflora Wallich. ex Baker
Article Sidebar
Main Article Content
Abstract
This research compared the different extraction methods (maceration, sonication, and microwave) with the ratio of ethanol and water (100:0, 80:20, 60:40, 40:60, 20:80, and 0:100) on yield of extraction, contents of total phenolic, and flavonoid in Kaempferia parviflora Wallich. ex Baker. The results showed that the yield of extraction, total phenolic compound, and flavonoid contents in Kaempferia parviflora Wallich. ex Baker had a significantly difference (P<0.05) on different extraction methods with the ratios of ethanol and water. The maximum yield (12.23±1.03 %) of the crude extract was observed at the maceration method with an ethanol to water ratio of 60:40 while the maximum total phenolic compound and flavonoid content were obtained by the microwave extraction method. The highest total phenolic compound (65.60±4.62 µgGAE/ mg extract) was obtained when extracted with ethanol to water ratio of 60:40. Furthermore, using ethanol to water ratio of 100:0 yielded the highest flavonoid content with 93.43±9.78 µgQE/ mg extract.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
กาญจนา นาคประสม จตุรภัทร วาฤทธิ์ อุมาพร อุประ หยาดฝน ทนงการกิจ และ นักรบ นาคประสม. 2560. สภาวะที่เหมาะสมในการสกัดสารประกอบฟีนอลิกรวมจากดอกบัวหลวง โดยใช้เทคนิคสกัดด้วยไมโครเวฟ. วารสารวิทยาศาสตร์ มข. 45(2): 328-342.
Azuma, T., Y. Tanaka and H. Kikuzuki. 2008. Phenolic glycosides from Kaempferia parviflora. Phytochemistry. 69(15): 2743-2748.
Baghdikian, B., A. Filly, A.S.F. Tixier, E. Petitcolas, F. Mabrouki, E. Chemat, and E. Ollivier. 2016. Extraction by solvent using microwave and ultrasound-assisted techniques followed by HPLC analysis of Harpagoside from Harpagophytum procumbens and comparison with conventional solvent extraction methods. CR. CHIM. 19(6): 692-698.
Chang, C. H., H.Y. Lin, C.Y. Chang and Y.C. Liua. 2006. Comparisons on the antioxidant properties of fresh, freeze-dried and hot-air-dried tomatoes. J. Food. Eng. 77: 478-485.
Chivapat, S., P. Chavalittumrong, A. Attawish and A. Rungsipipat. 2004. Chronic toxicity study of Kaempferia parviflora Wall ex. extract. Thai. J. Vet. Med. 40(4): 377-383.
Dent, M., V.D. Uzelac, M. Penic, M. Branic, T. Bosiljkov and B. Levaj. 2013. The effect of extraction solvents, temperature and time on the composition and mass fraction of polyphenols in Dalmatian Wild Sage (Salvia officinalis L.) extracts. Food. Technol. Biotechnol. 51(1): 84–91.
Do, Q.D., A.E. Angkawijaya, P.L. Tran-nguyen, L.H. Huynh, F.E. Soetaredjo, S. Ismadji and Y.H. Ju. 2014. Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatic. J. Food. Drug. Anal. 22(3): 296-302.
Eungpinichpong, W., U. Chatchawan, B. Sripanidkulchai, S. Arunpongpaisal and W. Chompoopan. 2018. Effects of Kaempferia parviflora on physical and psychological stresses in audits. Int. J. GEOMATE. 15(50): 26-31.
Golmakani, M.T. and K. Rezaei. 2018. Comparison of microwave-assisted hydro distillation with the traditional hydro distillation method in the extraction of essential oils from Thymus vulgaris L. Food Chemistry. 109(4): 925-930.
Hikmawanti, N.P.E., S. Fatmawati and A.W. Asri. 2021. The effect of ethanol concentrations as the extraction solvent on antioxidant activity of Katuk (Sauropus androgynus (L.) Merr.) leaves extracts. IOP Conf. Ser. Earth Environ. Sci. 755: 1-7.
Kkeiman, M., K.A. Ryu and A.P.E. Kahn. 2016. Determination of factors influencing the wet etching of polydimethylsiloxane using tetra-n-butyl ammonium fluoride. Macromol. Chem. Phys. 217: 284-291.
Lianfu, Z. and L. Zelong. 2008. Optimization and comparison of ultrasound/microwave assisted extraction (UMAE) and ultrasonic assisted extraction (UAE) of lycopene from tomatoes. Ultrason. Sonochem. 15: 731-737.
Liu, J.L., L.Y. Li and G.H. He. 2016. Optimization of microwave-assisted extraction conditions for five major bioactive compounds from flos sophorae immaturus (Cultivars of Sophora japonica L.) using response surface methodology. Molecules. 21(296): 1-27.
Mandal, V., Y. Mohan and S. Hemalatha. 2007. Microwave assisted extraction – An innovative and promising extraction tool for medicinal plant research. Pharmacogn. Rev. 1(1): 7-18.
Masota, N.E., G. Vogg, E. Heller and U. Holzgrabe. 2020. Comparison of extraction efficiency and selectivity between low-temperature pressurized microwave-assisted extraction and prolonged maceration. Arch. Pharm. 353(10): 1-11.
Prommajak, T., S. Surawang and N. Rattanapanone. 2014. Ultrasonic-assisted extraction of phenolic and antioxidative compounds from lizard tail (Houttuynia cordata Thunb.). Songklanakarin. J. Sci. Technol. 36(1): 65-72.
Rabeta, M.S. and M. Vithyia. 2013. Effect of different drying methods on the antioxidant properties of Vitex negundo Linn.Tea. Int. Food. Res. J. 20(6): 3171-3176.
Rosa, G.S., S.K. Vanga, Y. Gariepy and V. Raghavan. 2019. Comparison of microwave, ultrasonic and conventional techniques for extraction of bioactive compounds from olive leaves (Olea europaea L.). Innov. Food. Sci. Emerg. Technolies. 58: 1-8.
Saokaew, S., P. Wilairat, P. Raktanykan, P. Dilokthornsakul, T. Dhippayom, C. Kongkaew, R. Sruamsiri, A. Chuthaputti and N. Chaiyakunapruk. 2017. Clinical effects of krachaidum (Kaempferia parviflora): A systematic review. Evid. Based. Complement. Alternat. Med. 22(3): 413-428.
Siramon, P., T. Wongsheree and S. Yuadyong 2020. Ultrasound-assisted extraction of phenolic compounds from coconut endocarp and its radical scavenging activity. Naresaun. Phayao. J. 13(3): 22-28.
Sudwan, P., K. Saenphet, S. Saenphet and S. Suwansirikul. 2006. Effect of Kaempferia parviflora Wall. Ex. Baker on sexual activity of male rats and its toxicity. Southeast Asian J. Trop. Med. Public Health. 37(suppl 3): 210-215.
Sutthanut, K., B. Sripanidkulchai, C. Yenja and M. Jay. 2007. Simultaneous identification and quantitation of 11 flavonoid constituents in Kaempferia parviflora by gas chromatography. J. Chromatogr. A. 1143: 227–233.
Ueda, Y., N. Apiphuwasukcharoen, S. Tsutsumi, Y. Matsuda, V. Areekul and S. Yasuda. 2019. Optimization of hot-water extraction of dried yacon herbal tea leaves: enhanced antioxidant activities and total phenolic content by response surface methodology. Food. Sci. Technol. Res. 25(1): 131-139.
Vinatoru, M., J. T. Mason and I. Calinescu. 2017. Ultrasonically assisted extraction (UAE) and microwave assistedextraction (MAE) of functional compounds from plant materials. Trends. Analyt. Chem. 97: 150-178.
Wongsrikaew, N., H. Kim, K. Vichitphan, S.K. Cho and J. Ha. 2012. Antiproliferative activity and polymethoxyflavone composition analysis of Kaempferia parviflora extracts. J. Korean. Soc. Appl. Biol. Chem.55: 813−817.
Yenjai, C., K. Prasanphen and S. Daodee, V. Wongpanich and P. Kittakoop. 2004. Bioactive flavonoids from Kaempferia parviflora. Fitoterapia. 75(1): 89-92.
Zhang, Q.W., L.G. Lin and W.C. Ye. 2018. Techniques for extraction and isolation of natural products: a comprehensive review. Chin. Med. 13(20): 1-26.