Role of Glycinebetaine, Calcium and Potassium on Induction of Heat Tolerance in Tomato Seedlings
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Abstract
Supra-optimal temperature induces heat stress in plants which in turn affect quality and quantity of agricultural products negatively. Induction of heat tolerance by exogenous application of Glycinebetaine (GB) is an alternative approach which can alleviate the effect of heat stress during plant cultivation. However, the mechanism of GB on induction of heat tolerance is not well understood. This study investigated the involvement of calcium on the mechanism of GB using calcium channel blocker, Verapamil (VP), and potassium channel blocker, Tetraethylammonium Chloride (TEA) and tomato seedlings as a plant model. Results showed that tomato seedlings received foliar application of GB had higher photosynthetic rate, stomatal conductance, maximum quantum efficiency of PSII, quantum efficiency of PSII fresh weight and dry weight than the control received only water. Moreover, application of GB resulted in lower amount of Malondialdehyde and percentage of ion leakage of leaf tissue when compared to the control under heat stress condition. Application of either calcium or potassium channel blockers negated all the positive effects of GB. Results implied that the mechanism of GB-induced heat tolerance could be associated with the change of cellular concentration of calcium and potassium.
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