The Role of Microorganisms, Ash Quality and Hom’s Dye Process Containers in Indigo’s Ability to Change Colors During the Production of Natural Dye for the Production of Dye Sustainable

Abstract

     The purpose of this research was to study microorganisms involve in processing of Hom’s natural dye isolated from Kaewwanna (KW), Natural Fabric (TC), Hong Hom (HH), Pa Leung (PL) and Pa Ngiam (PG)shops. Ninety-five isolates of microoganisms were found but only 17 isolates were able to change Indigo blue to Leuco–indigo, especially TC228 from Natural Fabric shop provided the largest quantity of Leuco–indigo up to 9.5688 μg/ml and was classified in short gram–positive bacteria. After identifying species of TC228 by the nucleotide sequence of 16S rRNA, it was 99% identical to Bacillus cereus NR_115714.1 at length 1535 bp. To investigate the efficiency of Leuco–indigo changing, revealed that the sample added with TC228 provided more Leuco–indigo than sample without TC228 supplement. Moreover, the experimentation of appropriate containers for Hom’s natural dye process was not statistically significant differences. And the experimentation of lye quality from filtered wood ashes found that mixed wood ashes provided the highest Leuco–indigo up to 157.08 μg/ml. Color quality in L* a* and b* value are equal to 1.030 -0.023 and 0.367, respectively. According to the results of this research, it can make convenience and quality control also enhancing to the new style products e.g. Hom’s dye powder or Hom’s dye kit.