Evaluation of Hom (Strobilanthes spp.) Genotypes Using Molecular Techniques for In Situ Conservation in Phrae Province
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Abstract
This study aimed to identify species and assess genetic diversity of indigo plants (Hom) in Phrae province by integrating botanical traits with chloroplast DNA sequence analysis of the psbA–trnH region. Six samples collected from four localities were cultivated under controlled conditions for eight months to enable systematic morphological comparison. The results revealed clear differences in leaf color and size, branching pattern, plant height, and floral traits, allowing classification of SP1 - SP2 as Strobilanthes cusia, SP3 as closely related to Baphicacanthus cusia, and SP4 as Strobilanthes auriculata. Sequence analysis of the psbA–trnH region supported the morphological classification by clustering the samples into three distinct groups and showing 97 - 99% similarity with reference sequences in GenBank. These findings indicate that the psbA–trnH marker is effective for species discrimination and genetic relationship assessment in indigo plants. The generated data provides a useful molecular reference for genetic conservation and for future selection of high-quality indigo resources.
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References
Chanchay, N. 2021. Mho Hom Phrae: Wisdom and innovation for sustainable development. Smart Coating and Service Co., Ltd. [in Thai]
Chanchay, N. and A. Boonprajaub. 2021. Effect of pH on growth and indigo coloring quality of Hom (Baphicacanthus cusia (Nees.) Bremek.) at Phrae province. Thai Forest Ecological Research Journal 5(1): 91-104. Available: https://kuojs.lib.ku.ac.th/index.php/tferj/article/view/6145 [in Thai]
Chanchay, N. 2022. Manual for enhancing the potential and developing the process of planting and natural indigo dyeing with bio-innovation to elevate the economy in line with the new normal lifestyle in the Thung Hong subdistrict community, Mueang district, Phrae province. University to Tambon Project: Thung Hong subdistrict. Maejo University, Phrae. [in Thai]
Chanchay, N., P. Tagerngkool and A. Boonprajoub. 2024. Some environmental factors in each season of Hom (Strobilanthes cusia) production quality in Mueang district, Phrae province. Maejo Journal of Agricultural Production 6(2): 83-91. Available: https://li01.tci-thaijo.org/index.php/japmju/article/view/264369 [in Thai]
Hu, Y., D. Ma, S. Ning, Q. Ye, X. Zhao, Q. Ding, P. Liang, G. Cai, X. Ma, X. Qin and D. Wei. 2021. High-quality genome of the medicinal plant Strobilanthes cusia provides insights into the biosynthesis of indole alkaloids. Frontiers in Plant Science 12: 742420. Available: https://doi.org/10.3389/fpls.2021.742420.
Kress, W. J., K. J. Wurdack, E. A. Zimmer, L. A. Weigt and D. H. Janzen. 2005. Use of DNA barcodes to identify flowering plants. Proceedings of the National Academy of Sciences 102(23): 8369-8374. Available: https://doi.org/10.1073/pnas.0503123102.
Kumar, S., G. Stecher and K. Tamura. 2016. MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Molecular Biology and Evolution 33(7): 1870-1874. Available: https://doi.org/10.1093/molbev/msw054.
Pang, X., C. Liu, L. Shi, R. Liu, D. Liang, H. Li, S. S. Cherny and S. Chen. 2012. Utility of the trnH–psbA intergenic spacer region and its combinations as plant DNA barcodes: A meta-analysis. PLoS ONE 7(11): e48833. Available: https://doi.org/10.1371/journal.pone.0048833.
Teanglum, A., S. Teanglum and A. Saithong. 2012. Selection of indigo plant varieties and other plants that yield indigo dye. Procedia Engineering 32: 184-190. Available: https://doi.org/10.1016/j.proeng.2012.01.1255.
Wang, T., X. Li, C. Tang, Z. Cao, H. He, X. Ma, Y. Li and K. De. 2024. Complete chloroplast genomes and phylogenetic relationships of Pedicularis chinensis and Pedicularis kansuensis. Scientific Reports 14: 14357. Available: https://doi.org/10.1038/s41598-024-63815-0.
Zhang, D., J. Tu, X. Ding, W. Guan, L. Gong, X. Qiu, Z. Huang and H. Su. 2023. Analysis of the chloroplast genome and phylogenetic evolution of Bidens pilosa. BMC Genomics 24: 113. Available: https://doi.org/10.1186/s12864-023-09195-7.
