KINETIC OF NITRATE REDUCING BACTERIA FOR NHAM PRODUCTION

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Pairote Wiriyacharee
Lakkana Rujanakraikarn
Wiwat Wattanatchariya
Suthaya Boonthanom

Abstract

Micrococcus varians is a nitrate reducing bacteria which needs glucose as a carbon substrate for cell growth and its activity. Therefore, glucose was used for kinetics study in the model system. For this experiment, glucose was varied as studied factor to 0.22-0.40% for M.varians at 48 hours of fermentation period. It was found that the maximum specific growth rate (um) of M.varians was 0.2534 hr-1 and saturation constant (Ks) was about 0.1799%. However, M.varians can produce a few of lactic acid content. As a result of this, the pH was also higher. In the nitrate reduction activity, it can reduce nitrate to nitrite with amount of 80% in the first period of 12-18 hours. But the reduction of nitrate was dependent upon the nitrate level in the system.

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References

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