การผลิตเอนไซม์และฤทธิ์ต้านเชื้อราของแบคทีเรียปฏิปักษ์ <I>Streptomyces cavourensis</I> BUU135
Article Sidebar
Main Article Content
บทคัดย่อ
Streptomyces spp. สามารถผลิตสารเมแทบอไลต์ทุติยภูมิและสารปฏิชีวนะหลายชนิด นำไปสู่วัตถุประสงค์ของการศึกษาในครั้งนี้ เพื่อทดสอบความสามารถของ Streptomyces cavourensis BUU135 ในการ-ผลิต extracellular enzyme และประเมินความสามารถในการยับยั้งเชื้อรา จากผลการศึกษาพบว่า S. cavourensis สามารถละลายฟอสเฟต และผลิตเอนไซม์เซลลูเลส อะไมเลส ยูรีเอส ไคติเนส โปรตีเอส แคทาเลส และไซเดอร์โรฟอร์ได้ นอกจากนี้ S. cavourensis ยังสามารถยับยั้งการเจริญของเชื้อราก่อโรคพืช ได้แก่ Phytophthora palmivora, Fusarium decemcellulare และ Lasiodiplodia theobromae (การยับยั้ง 44.3, 41.5 และ 61.1 เปอร์เซ็นต์ ตามลำดับ) จากผลการศึกษาเหล่านี้บ่งชี้ได้ว่า S. cavourensis เป็นผู้ผลิตสารเมแทบอไลต์ทุติยภูมิที่ออกฤทธิ์ต่อจุลินทรีย์ชนิดอื่น โดยเฉพาะเชื้อราได้เป็นอย่างดี และมีแนวโน้มในการใช้เพื่อควบคุมราโรคพืชในดินได้หลากหลาย
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
Agrawal, T. and A.S. Kotasthane. 2012. Chitinolytic assay of indigenous Trichoderma isolates collected from different geographical locations of Chhattisgarh in Central India. SpringerPlus 1(1): 73, doi: 10.1186/2193-1801-1-73.
Alnahdi, H.S. 2012. Isolation and screening of extracellular proteases produced by new Isolated Bacillus sp. Journal of Applied Pharmaceutical Science 2(9): 71-74.
Fróes, A., A. Macrae, J. Rosa, M. Franco, R. Souza, R. Soares and R. Coelho. 2012. Selection of a Streptomyces strain able to produce cell wall degrading enzymes and active against Sclerotinia sclerotiorum. The Journal of Microbiology 50(5): 798-806.
Ghanem, G.A.M., D.A.S. Gebily, M.M. Ragab, A.M. Ali, N.E.D. Soliman and T.H. Abd El-Moity. 2022. Efficacy of antifungal substances of three Streptomyces spp. against different plant pathogenic fungi. Egyptian Journal of Biological Pest Control 32: 112, doi: 10.1186/s41938-022-00612-9.
González, V., M.J. Vargas-Straube, W.O. Beys-da-Silva, L. Santi, P. Valencia, F. Beltrametti and B. Cámara. 2022. Enzyme bioprospection of marine-derived actinobacteria from the Chilean coast and new insight in the mechanism of keratin degradation in Streptomyces sp. G11C. Marine Drugs 18(11): 537, doi: 10.3390/md18110537.
Gottlieb, D. 1967. Flavensomycin. pp. 617-620. In: D. Gottlieb and P.D. Shaw (eds.). Antibiotics Volume 1: Mechanism of Action. Springer-Verlag, Berlin.
Hwang, K.-S., H.U. Kim, P. Charusanti, B.Ø. Palsson and S.Y. Lee. 2014. Systems biology and biotechnology of Streptomyces species for the production of secondary metabolites. Biotechnology Advances 32(2): 255-268.
Kaaniche, F., A. Hamed, L. Elleuch, A. Chakchouk-Mtibaa, S. Smaoui, I. Karray-Rebai, I. Koubaa, G. Arcile, N. Allouche and L. Mellouli. 2020. Purification and characterization of seven bioactive compounds from the newly isolated Streptomyces cavourensis TN638 strain via solid-state fermentation. Microbial Pathogenesis 142: 104106, doi: 10.1016/j.micpath.2020.104106.
Kafilzadeh, F. and F. Dehdari. 2015. Amylase activity of aquatic actinomycetes isolated from the sediments of mangrove forests in south of Iran. The Egyptian Journal of Aquatic Research 41(2): 197-201.
Kaur, T., R. Rani and R.K. Manhas. 2019. Biocontrol and plant growth promoting potential of phylogenetically new Streptomyces sp. MR14 of rhizospheric origin. AMB Express 9(1): 125, doi: 10.1186/s13568-019-0849-7.
Lacey, H.J. and P.J. Rutledge. 2022. Recently discovered secondary metabolites from Streptomyces species. Molecules 27(3): 887, doi: 10.3390/molecules27030887.
Le, K.D., J. Kim, H.T. Nguyen, N.H. Yu, A.R. Park, C.W. Lee and J-C. Kim. 2021. Streptomyces sp. JCK-6131 protects plants against bacterial and fungal diseases via two mechanisms. Frontiers in Plant Science 12: 726266, doi: 10.3389/fpls.2021.726266.
Lee, S.Y., H. Tindwa, Y.S. Lee, K.W. Naing, S.H. Hong, Y. Nam and K.Y. Kim. 2012. Biocontrol of anthracnose in pepper using chitinase, β -1,3 glucanase, and 2-furancarboxaldehyde produced by Streptomyces cavourensis SY224. Journal of Microbiology and Biotechnology 22(10): 1359-1366.
Malviya, M.K., P. Trivedi and A. Pandey. 2018. Chitinase and glucanase activities of antagonistic Streptomyces spp. isolated from fired plots under shifting cultivation in northeast India. Journal of Advanced Research in Biotechnology 3(1): 1-7.
Manullang, W. and H. Chuang. 2020. Streptomyces sp. mitigates abiotic stress response and promotes plant growth. Journal of Plant Protection Research 60(3): 263-274.
Morou-Bermudez, E. and R.A. Burne. 2000. Analysis of urease expression in Actinomyces naeslundii WVU45. Infection and Immunity 68(12): 6670-6676.
Nguyen, H.Q., N.T.-H. Vu, H.H. Chu, S.K. Chu, H. Hoang, T.T. Tran, C. Nguyen, L.T.-M Dinh, H.T.-T Trinh and T.Q. Phi. 2018. Draft genome sequence of Streptomyces cavourensis YBQ59, an endophytic producer of antibiotics bafilomycin D, nonactic acid, prelactone B, and
,11-epoxy-10-cadinanol. Microbiology Resource Announcements 7(11): 201056-18, doi: 10.1128/MRA.01056-18.
Nimisha, P., S. Moksha and A.K. Gangawane. 2019. Amylase activity of starch degrading bacteria isolated from soil. International Journal of Current Microbiology and Applied Sciences 8(4): 659-671.
Pacios-Michelena, S., C.N. Aguilar González, O.B. Alvarez-Perez, R. Rodriguez-Herrera, M. Chávez-González, R. Arredondo Valdés, J.A. Ascacio Valdés, M. Govea Salas and A. Ilyina. 2021. Application of Streptomyces antimicrobial compounds for the control of phytopathogens. Frontiers in Sustainable Food Systems 5: 696518, doi: 10.3389/fsufs.2021.696518.
Pradhan, A., A. Pahari, S. Mohapatra and B.B. Mishra. 2017. Phosphate-solubilizing microorganisms in sustainable agriculture: Genetic mechanism and application. pp. 81-97. In: T. Adhya, B. Mishra, K. Annapurna, D. Verma and U. Kumar (eds.). Advances in Soil Microbiology: Recent Trends and Future Prospects. Springer, Singapore.
Pragya, R., A. Yasmin and J. Anshula. 2012. An insight into agricultural properties of actinomycetes. International Journal of Research in Biosciences 1(1): 7-12.
Quinn, G.A., A.M. Banat, A.M. Abdelhameed and I.M. Banat. 2020. Streptomyces from traditional medicine: Sources of new innovations in antibiotic discovery. Journal of Medical Microbiology 69(8): 1040-1048.
Sheik, G.B., A.A. Alhumaidy, A.I.A. Abdel Raheim, Z.A. Alzeyadi and M.I. AlGhonaim. 2020. Taxonomic characterizations of soil Streptomyces cavourensis DW102 and its activity against fungal pathogens. Journal of Pharmacy and Bioallied Sciences 12(4): 462-467.
Shrivastava, P., R. Kumar, M.S. Yandigeri, N. Malviya and D.K. Arora. 2015. Isolation and characterization of Streptomycetes with plant growth promoting potential from mangrove ecosystem. Polish Journal of Microbiology 64(4): 339-349.
Siddharth, S., R.R. Vittal, J. Wink and M. Steinert. 2020. Diversity and bioactive potential of actinobacteria from unexplored regions of Western Ghats, India. Microorganisms 8(2): 225, doi: 10.3390/microorganisms 8020225.
Singh, L.S., I. Baruah and T.C. Bora. 2006. Actinomycetes of Loktak habitat: Isolation and screening for antimicrobial activities. Biotechnology 5(2): 217-221.
Sirikun, B. and N. Thaochan. 2018. Chitinase and protease production from indigenous insecticidal actinomycetes varieties. Khon Kaen Agriculture Journal 46(Suppl.1): 739-744. (in Thai)
Su, S.-S., L. Tian, G. Chen, Z.-Q. Li, W.-F. Xu and Y.-H. Pei. 2013. Two new compounds from the metabolites of a marine-derived actinomycete Streptomyces cavourensis YY01-17. Journal of Asian Natural Products Research 15(3): 265-269.
Sultana, S., S. Alam and M.M. Karim. 2021. Screening of siderophore-producing salt-tolerant rhizobacteria suitable for supporting plant growth in saline soils with iron limitation. Journal of Agriculture and Food Research 4: 100150, doi: 10.1016/j.jafr.2021.100150.
Tanatip, C. and K. Kunasakdakul. 2017. Paper type bioproduct of endophytic actinomycetes for controlling bakanae disease of Riceberry rice in seedling stage. Journal of Agriculture 34(1): 67-75. (in Thai)
Tangwattanachuleeporn, M., P. Ruangsuj, W. Yamprayoonswat, S. Sittihan, W. Jumpathong and M. Yasawong. 2021. Genome sequence of Streptomyces cavourensis BUU135, isolated from soil from a tropical fruit farm in Thailand. Microbiology Resource Announcements 10: 19, doi: 10.1128/MRA.01428-20.
Wilson, D.B. 2009. Cellulases. pp. 252-258. In: M. Schaechter (ed.). Encyclopedia of Microbiology. Elsevier/Academic Press, Amsterdam.
Yodpanan, P. and P. Thapanapongworakul. 2019. Selection of endophytic actinomycetes against Phytophthora nicotianae causing leaf blight and fruit rot of passion fruit. Khon Kaen Agriculture Journal 47 (Suppl. 1): 1633-1638. (in Thai)