Effects of Hemp Extract Supplementation in Frozen Bull Semen Extender on Post-Thaw Sperm Quality
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Abstract
The purpose of this study was to investigate the effects of hemp extract supplemented in frozen bull semen extender on post-thaw sperm quality. The control group consisted of an egg yolk-based basic semen diluent (Tris-egg yolk: EG). Two experiments were divided, experiment 1, tested the changes in osmotic pressure values of hemp extract diluent containing CBD at levels of 5, 10, 50, and 100 µg/mL, respectively. The retention time in the preservation of the osmosis diluent at 4 oC was 0, 12, 24, 48, 72, 96, 120, 144 hours, and 1 week. It was found that the osmotic pressure values of all experimental groups were optimal for the freezing of bull semen and had no effect on the quality of the frozen bull semen. Experiment 2, the supplementation of hemp extract in frozen bull semen diluent on sperm quality after thawing at concentrations of 5, 10, 50, and 100 µg CBD/mL, respectively, was tested in 3 Tropical Holstein (TH) dairy cows. Twenty million sperm per tube and packed into 0.25 mL ministraw tubes, incubated at 4 and -121 oC for 4 hours and 15 minutes, respectively, and stored in liquid nitrogen at -196 oC. After that, semen quality was assessed by computer-assisted sperm analysis (CASA) at 24 hours, 1 week, 2 weeks, and 1 month after cryopreservation. It was found that 5 µg CBD/mL hemp extract supplementation affected sperm motility (49.43 %) significantly more than the control group (P < 0.05). Hemp extract supplementation at a CBD level of 5 µg/mL in semen dilution found sperm with a bent tail and proximal droplet were significantly lower than the control group (P < 0.05). In conclusion, the use of a hemp extract supplementation in frozen bull semen extender level at 5 µg CBD/mL showed good sperm quality tendencies. It is an adjuvant in the supplementation of frozen bull semen.
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