Genotypic Effects of Insulin-like Growth Factor Binding Protein 2 on Fat-free Lean Traits in Crossbred between Duroc and Pietrain Pigs
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Abstract
Insulin-like growth factor binding protein 2 (IGFBP 2) was selected as a candidate gene for economically important traits in pigs. For this study, 11 phenotypic data of fat-free lean at several ages were collected from 408 live-F2 pigs obtained from Duroc × Pietrain MSU resource population. The study traits included fat-free lean, fat- free lean percent at ages 10, 13, 16, 19 and 22 weeks as well as fat-free lean gain from 10 to 22 weeks. Three IGFBP 2 genotypes (AA, AB and BB) were determined using MspI PCR-RFLP. The statistical model, which included the fixed effects of IGFBP 2 genotypes and sex, as well as the random effects of the farrowing group, litter and finishing pen nested within the farrowing group and residual error, was used to determine potential associations of the IGFBP 2 genotypes with fat-free lean traits. The results indicated a significant effect (P<0.05) of the IGFBP 2 genotypes on these traits. Pigs with the BB genotype had more favorable (P<0.05) fat-free lean at 10, 13 and 16 weeks of age than pigs with the AB genotype, but there was non-significant effect (P>0.05) of the IGFBP 2 genotypes on fat-free lean at 19 and 22 weeks of age. Furthermore, pigs with the BB genotype had most favorable (P<0.05) fat-free lean percent at 13, 16, 19 and 22 weeks of age as well as fat-free lean gain from 10 to 22 weeks. The results of this study indicated associations between the IGFBP 2 genotypes and fat-free lean traits in F2 crossbred pigs, and the validation of these results in additional breeds and pig populations is warranted.
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