Resistant Induction of Phytophthora Root Rot in Strawberry Tissue Culture Plantlets Using Endophytic Actinomycetes
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Abstract
Ninety-seven isolates of endophytic actinomycetes isolated from Rosaceae and herbal plants were used to evaluate their inhibitory effects on growth of Phytophthora cactorum root rot pathogen of strawberry var. 329 (Yeal). Percent inhibitions of the radial growth (PIRG) of the strawberry root rot fungi were measured after dual culture method was done compared to control treatment. Five isolates of endophytic actinomycetes; DUC2, CINv1, FRA19, CINc1 and POL2, were selected since there were non-significant in inhibitory levels of the PIRG at 95.51, 94.38, 93.43, 89.89 and 88.96, respectively. Strawberry tissue culture plantlets were further proved for colonization by these five endophytic actinomycetes using re-isolation method. The results found that all isolates successfully colonized in plant tissue particularly isolate DUC2 showed the highest colonization with the highest re-isolation percentages at 96. Furthermore, the colonized strawberry tissue culture plantlets were proved for their root rot resistant induction after the pathogen oospores were inoculated onto the plants. The disease severity data revealed that, in vitro plantlets colonized with the isolate CINv1 and FRA19 were the best trials to induce root rot resistance. There were non-significant disease severity levels determined at 2.20 and 2.50, respectively compared to the level at 3.90 of the uncolonized control trial.
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