Effects of Electrolyzed Oxidizing Water on Powdery Mildew Reduction of Cucurbits
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Abstract
The purpose of this study was to examine the effects of electrolyzed oxidizing water (EO water) on inhibition of conidial germination and reduction of powdery mildew fungi caused by Fibroidium sp. in Cucurbits. Conidia of powdery mildew fungi were collected from naturally infected plants then maintained on inoculated Japanese cucumber seedlings in vitro before being inducted and identified. EO water was produced using Super Oxseed Labo (wonder BJ F850) at 6 concentrations of 0.05, 0.1, 0.2, 0.3, 0.4 and 0.5 percent NaCl solutions. The pH and electrolyte conductivity (EC) were measured and showed at the range of 3.71-3.93 and 1.81-4.13 mS/cm, respectively. In addition, the available free chlorine concentrations were also determined with N, N-diethyl-p-phenylenediamine (DPD) testing and showed at the range of 18.7-165 ppm. Testing for the effect on conidia germination of Fibroidium sp. was done by spraying EO water on brushing conidial agar discs and incubated for 12 and 24 hrs. at 25 ºC. The results showed that conidial germinations were completely inhibited when treated with EO water at the concentration of 0.1-0.5 percent. Inhibition rate of conidial germination treated by 0.05 percent EO water and distilled water were 67.78 and 71.11 percent, respectively. However, phytotoxic symptom affected from EO waters were determined on Japanese cucumber and found that after spraying for 3 days, shoot tip of treated plants was burnt when treated with EO water at 0.4-0.5 percent. The EO water at 0.3 percent is able to reduce disease incidence for 29.70 percent. In addition, enlargement of fungal colony size decreased and the mycelial growth and conidiophores were collapsed. The density of conidia per colony significantly reduced after spraying with each EO water concentration compared to untreated control (distilled water).
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