Using of Soil Actinomycetes to Inhibit <I> Ralstonia solanacearum </I> Causal Agent of Tomato Wilt Disease
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Abstract
Actinomycetes were isolated from soil surrounding tomato root which collected from tomato plantation areas of Inthanon Royal Agricultural Station and Khun Wang Royal Project Development Center. A total of 126 actinomycetes isolates were obtained and used for evaluation of antagonistic activity against Ralstonia solanacearum (causes bacterial wilt disease of tomato) by using dual culture method on NA medium. Seventeen actinomycetes isolates demonstrated an ability to inhibit growth of R. solanacearum. The highest antagonistic activity are shown by T-11 isolate with 16.50 mm of inhibition zone. Moreover, T-11 isolate could produce siderophore and cellulase enzyme under agar plate assay. Identification of isolate T-11 based on 16S rRNA gene sequencing was analyzed to confirm the species. The result showed that partial gene sequencing of T-11 isolate was 96% homology with Streptomyces mirabilis.
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