Seasonal Expression Lavels of Vitellogenin Gene and Gonadosomatic Index in <I> Pristolepis fasciata </I> in Kwan Phayao, Phayao Province
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Abstract
The aims of this study were to amplify the partial cDNA sequence of vitellogenin gene (VTG) for determination the VTG seasonal expression and gonadosomatic index (GSI) of female Pristolepis fasciata. The sample fishes were collected from Kwan Phayao for 1 year period between June 2012 and May 2013. The 320-bp fragment of P. fasciata VTG (called PfVTG) type Ab was amplified from the liver tissue of female fish using RT-PCR technique and VF1 and VR1 primers. A phylogenetic tree based on three types of VTG, including Aa, Ab and C from other teleost fishes was constructed for relationship analysis. The nodes were separated into 3 major branches following the type of VTG. The PfVTG was grouped into the VTG type Ab. The seasonal expression levels of VTG were determined using real-time PCR. The VTG expression levels and GSI were significantly (P<0.05) peaked in 2 periods, July and January. This result showed that the P. fasciata in Kwan Phayao could spawn one more time per year. The correlation analysis showed the VTG expression levels have a significant (rs= 0.58; P<0.01) direct relation with the GSI. From these results indicated that apart from the GSI, the seasonal expression of VTG could also reflect the spawning season of P. fasciata in Kwan Phayao.
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