Effect of Sucrose on Growth and In Vitro Tuberization of Pecteilis sagarikii Seidenf.

Abstract

Effect of sucrose concentrations on growth and in vitro tuberization of Pecteilis sagarikii Seidenf. was investigated. Protocorms derived from in vitro seed germination were transferred onto CMU1 medium adding sucrose concentration at   0,  1,  2,  3,  5 and 7 % (w/v). It was found that increasing sucrose concentrations has no significant effect on shoot height and number of leaf per plant, but resulting in slow leaf growth and expansion, also decreasing in leaf size. For tuber formation, it started within one week after culturing. The highest concentration of sucrose at 7 % affected in slow tuberization and decreased in tuberization percentage. Increasing sucrose concentration has no significant positive effect on number of tuber per plant, but resulting in significant decrease in tuber size. The tuber grew well and produced the biggest size when cultured on the medium adding 1 % sucrose.  Sucrose concentration has no pronounced significant effect on number of root, root width and root length.