<I>Agrobacterium</I>-Mediated Transformation of <I>Cry1Ac </I>Gene to Tobacco (<I>Nicotiana tabacum</I>) and Evaluation of <I> Heliothis armigera </I>Resistance
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Abstract
Agrobacterium mediated gene transfer is a standard technique in plant genetic engineering. For the gene transfer, leaf discs of tobacco variety K326 were co-cultured with A. tumefaciens strain EHA carried Cry1Ac, bar and GUS genes which are insect toxin, herbicide resistant and reporter genes respectively in the plasmid pITB2. Phosphinothricin (PPT)-resistant shoots that express GUS activity were derived from the cultured leaf discs and the presence of Cry1Ac was checked using PCR and toxin protein was determined by Western Blot analysis. The in vivo assay using cotton bollworm larvae showed that the transgenic plants were protected from the feeding and the loss became minimized.
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References
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