Effects of Electrical Energy Stimulation with Different Stimulation Time on Mycelium Growth and Physical Quality of Reishi Mushroom (<I>Ganoderma lucidum</I>)

Abstract

Effects of electrical stimulation of mycelium at various stimulation times on mycelium growth and physical quality of Reishi mushroom (Ganoderma lucidum) were studied. The results demonstrated that electrical stimulation at 100 kV caused the mycelium of Reishi mushrooms to grow faster on PDA than stimulation at 25 kV. When Reishi mushroom mycelium was treated with 100 kV electrical stimulation, prior to cultivating on millet seed medium, it grew vertically and horizontally faster than other treatments. Furthermore, it showed that the mycelium of Reishi mushroom treated with 50 kV electrical stimulation developed better on sawdust media than other treatments. When the mushrooms reached commercial maturity, Reishi mushroom stalk length grew from mycelium stimulated with 100 kV electrical energy was longer than stimulated with 25 kV electrical energy. Regarding the period of electrical stimulation, it was discovered that electrical stimulation for 30 seconds resulted in the best development of Reishi mushroom mycelium on PDA media. When the mycelium was applied to sorghum seed media, it showed that the length of electrical stimulation had no effect on mycelium growth, both vertical and horizontal. Furthermore, Reishi mushroom mycelium that had been electrically stimulated for 10 seconds grew more efficiently on sawdust than other treatments. However, the electrical energy level and period of electrically stimulating, the mycelium had no influence on the basidiocarp width, length, thickness, diameter of stalk, fresh weight, and dry weight of the Reishi mushroom.