<I>In Vitro<I> Propagation of Heracleum siamicum Craib

Abstract

Heracleum siamicum Craib. is herbal spice plant that contains many important chemical components. However, there are a few research information on its propagation by tissue culture. This research was aimed to study the optimal media for H. siamicum Craib. shoot induction. The 1 cm shoot tips were cultured on the MS and WPM media which supplemented with different of BAP and TDZ at concentrations of 0, 1, 2, 4 and 8 mg/L with 20 replicates for 45 days. The results showed that the BAP addition of any 2 mg/L gave significantly greater shoot number (5.55±0.87 shoots/explant) than the other (P<0.05). While, the MS and WPM media without growth regulators exhibited the greatest shoot length of 6.80±0.42 and 7.21±0.35 cm, respectively. The H. siamicum Craib. root induction was afterwards conducted on the MS and WPM media supplemented with different growth regulators of IBA, IAA and NAA at 0, 1, 2, 4 and 8 mg/L with 20 replicates. It was shown that the MS medium plus 1 mg/L IBA gave the highest percentage of root induction of 90%, higher root number of 15.65±2.06 roots per shoot and root length of 1.81±0.29 cm. H. siamicum Craib. shoot was induced callus formation of 100% and callus diameter of 2.43±0.13 cm on the WPM medium plus 8 mg/L NAA. The plantlets with both roots and shoots were transferred to various planting materials which were vermiculite, perlite, peat, and the mixture of vermiculite, perlite, and peat at the ratio of 1:1:1 for 30 days in the green house. It was found that H. siamicum Craib. planted in the mixtures of vermiculite, perlite, and peat had 50% survival percentage and average plant height 3.5 cm.