Callus Induction and Plantlet Regeneration from Anther of <I>Andrographis paniculata</I> (Burm.f.) Wall.ex Nees. <I>In Vitro</I>
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Abstract
In vitro anther culture of Andrographis paniculata (Burm.f.) Wall.ex Nees. from Chiang Mai for callus induction was studied. The flowers were harvested 9 to 7 days before blooming and were used for anther culture. The anther explants were cultured on Nitsch & Nitsch medium (1967), as modified by Lichter (1982) (NLN), supplemented with 0, 0.1, 0.5, and 1.0 mg/L 6-Benzylaminopurine (BAP) in combination with 0, 0.1, and 0.5 mg/L Naphthalene acetic acid (NAA) for 8 weeks. The results showed that the most effective treatment for callus induction was cultured on NLN medium supplemented with 0.5 mg/L NAA in combination with 1.0 mg/L BAP. In order to increase the callus and induce embryo, callus was cultured at the low temperature of 4°C for 1 and 3 days, it was further transplanted under high temperature of 28, 30 and 32°C for 1, 2, 3 and 4 days on NLN medium supplemented with 0.5 mg/L NAA in combination with 1.0 mg/L BAP. The results showed that the highest weight callus and induced somatic embryo were cultured at a low temperature of 4°C for 1 day and then transplanted at a high temperature of 28°C for 1 day could increase the quantity of callus and induce to form an embryo.
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