Effects of Indole-3-Butyric Acid and Gibberellic Acid on Adventitious Root Formation and Growth of ‘Akihime’ Strawberry Stolon
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Abstract
This study aimed to investigate root induction in daughter plants of the ‘Akihime’ strawberry grown in a modified CMU2S nutrient solution in an evaporative cooling greenhouse at the Faculty of Agriculture, Chiang Mai University. In the first week, the electrical conductivity (EC) of the nutrient solution was maintained at 0.0 mS/cm and gradually increased to 1.0, 1.5, and 2.0 mS/cm in the second, third, and fourth weeks, respectively. The experiment was conducted using a (2×2)+1 factorial arrangements in a completely randomized design (CRD) with seven replications. Factor A consisted of indole-3-butyric acid (IBA) at 0.6 and 1.2 mg/L while Factor B consisted of gibberellic acid (GA3) at 50 and 150 mg/L. The basal ends of the daughter plants were dipped in the respective treatment solutions for 3 min, with distilled water used as the control. The results revealed an interaction between IBA and GA3 on the number of roots and chlorophyll content in strawberry daughter plants. All treatments had a lower number of roots and chlorophyll content than the control, except for the combination of 1.2 mg/L IBA and 50 mg/L GA3, which showed no significant difference from the control.
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References
Ashrafuzzaman, M., S.M. Faisal, D. Yadav, D. Khanam and F. Raihan. 2013. Micropropagation of strawberry (Fragaria x ananassa) through runner culture. Bangladesh Journal of Agricultural Research 38(3): 467 - 472.
Bai, T., Z. Dong, X. Zheng, S. Song, J. Jiao, M. Wang and C. Song. 2020. Auxin and its interaction with ethylene control adventitious root formation and development in apple rootstock. Frontiers in Plant Science 11: 574881, doi: 10.3389/fpls.2020.574881.
De Klerk, G.J., W. Van Der Krieken, and J.C. de Jong. 1999. Review the formation of adventitious roots: new concepts, new possibilities. In Vitro Cellular & Developmental Biology-Plant 35(3): 189 -199.
De Rybel, B., D. Audenaert, W. Xuan, P. Overvoorde, L.C. Strader and S. Kepinski. 2012. A role for the root cap in root branching revealed by the non-auxin probe naxillin. Nature Chemical Biology 8(9): 798 - 805.
Edelmann, H.G. 2022. Plant root development is the classical theory for auxin-regulated root growth false?. Protoplasma 259(3): 823 - 832.
Frick, E.M. and L.C. Strader. 2018. Roles for IBA-derived auxin in plant development. Journal of Experimental Botany 69(2): 169 - 177.
Guan, L., A.S. Murphy, W.A. Peer, L. Gan, Y. Li. and Z.M. Cheng. 2015. Physiology and molecular regulation of adventitious root formation. Critical Reviews in Plant Sciences 34(5): 506 - 521.
Hancock, J.F. 2020. Strawberries (2nd ed.). CABI Publishing, Wallingford, Oxfordshire, United Kingdom 287 p.
Hossain, M.M. and O. Gony. 2020. Influence of indole butyric acid on root induction in daughter plants of strawberry. Journal of Applied Horticulture 22(3): 209 - 214.
Islam, M.S., M.A. Hoque, S.A. Sumi, M. Jony, S.A.R Kamrunnahar and M. Shamsuzzaman. 2020. Effects of foliar application of indole butyric acid (IBA), gibberellic acid (GA3) and zinc (Zn) on yield and quality of tomato. International Journal of Plant and Soil Science 32(1): 1 - 9.
Ivanchenko, M.G., S. Napsucialy-Mendivil and J.G. Dubrovsky. 2010. Auxin-induced inhibition of lateral root initiation contributes to root system shaping in Arabidopsis thaliana. The Plant Journal 64(5): 740 - 752.
Iyer-Pascuzzi, A.S. and P.N. Benfey. 2009. Transcriptional networks in root cell fate specification. Biochimica et Biophysica Acta (BBA)-Gene Regulatory Mechanisms 1789(4): 315 - 325.
Lakehal, A. and C. Bellini. 2019. Control of adventitious root formation: insights into synergistic and antagonistic hormonal interactions. Physiologia plantarum 165(1): 90 - 100.
Liu, H.J., K.Q. Tao, T.H. Shen, D.L. Song and C.Y. Wang. 2012. Semi-forcing cultivation of'Akihime'strawberry in Heilongjiang Province, China. In VII International Strawberry Symposium 1049 (pp. 517 -520).
Nishii, K., C.N. Wang, A. Spada, T. Nagata and M. Möller. 2012. Gibberellin as a suppressor of lateral dominance and inducer of apical growth in the unifoliate Streptocarpus wendlandii (Gesneriaceae). New Zealand Journal of Botany 50(3): 267 - 287.
Stern, R.A., M. Flaishman, S. Applebaum and R. Ben-Arie. 2007. Effect of synthetic auxins on fruit development of ‘Bing’cherry (Prunus avium L.). Scientia Horticulturae 114(4): 275 - 280.
Taiz, L., E. Zeiger, I.M. Møller and A. Murphy. 2015. Plant Physiology and Development (6thed.). Sinauer Associates. Sunderland, CT. 761 p.
Techapinyawat, S. 1993. Plant Physiology. Amarin Long Ting Group, Bangkok. 222 p. (in Thai)
Thongrod, N. 1990. Effect of gibberellin on rune production and growth of Tioga strawberry. M.S.Thesis, Kasetsart University, Bangkok. 46 p. (in Thai)
Tripathi, J.M., B.R Khan, R. Gaur, D. Yadav, K.K. Verma and R. Gupta. 2025. Gibberellic acid improves photosynthetic electron transport and stomatal function in crops that are adversely affected by salinity exposure. Plants 14(21): 3388, doi: 10.3390/plants14213388.
Zhang, J., T. Zhou, C. Zhang, W. Zheng, J. Li, W. Jiang, C. Xiao, D. Wei, C. Yang, R. Xu, A. Gong and Y. Bi. 2021. Gibberellin disturbs the balance of endogenesis hormones and inhibits adventitious root development of Pseudostellaria heterophylla through regulating gene expression related to hormone synthesis. Saudi Journal of Biological Sciences 28(1): 135 - 147.
Zhao, H., Y. Wang, S. Zhao, Y. Fu and L. Zhu. 2021. HOMEOBOX PROTEIN 24 mediates the conversion of indole-3-butyric acid to indole‐3‐acetic acid to promote root hair elongation. New Phytologist 232(5): 2057 - 2070.
