Optimization of Cellulose Production from Orange Pomace by <I>Acetobacter xylinum</I>
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Abstract
The objectives of this study were to determine the optimum concentration of mannitol (3 - 7%) and yeast extract (0.3 - 0.7%) as well as the optimum shaking rate (0, 50, 100 and 150 rpm) for cellulose production from orange pomace by Acetobacter xylinum and to examine other qualities of bacterial cellulose and marinade. It was found that the cultivation of A. xylinum on medium made from orange pomace supplemented with 3 % mannitol and 0.66% yeast extract gave the highest cellulose yield of 25.31 g (from 30 g of orange pomace). The addition of different amounts of mannitol and yeast extract affected the changes in color values, L*, a*, b* and moisture content of cellulose sheets, as well as reducing sugar content, soluble solids and acetic acid content of the marinade. While the optimum shaking rate was 100 rpm, the maximum cellulose yield was 42.82 g and the maximum acetic acid concentration in the marinade was found to be 2.93 g/L at the shaking rate of 50 rpm.
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