Impact of Extender pH on Motility of Bovine Sexed Semen by M-Zlex Process
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Abstract
Sperm motility is an effective indicator of sperm fertility, and environmental factors such as pH, osmolarity, and temperature can modify the function and motility of sperm. In this study, we evaluated the effect of different extenders pH values on the motility of bovine sexed semen. Semen samples were divided into two parts: conventional semen (CONV) diluted with different extenders pH 6.2, 6.6, 7.0, 7.4, and 7.8 and sexed semen by M- Zlex process (M- Zlex) that used different extenders pH 6.2, 6.6, 7.0, 7.4, and 7.8 during the sexing process. An imaging flow cytometer was used to evaluate X-sperm ratio, and CASA was used to analyze the motility of sperm after dilution (AD) and post-thaw semen (PT). The results showed an average of X-sperm for 50.06% in CONV, while M - Zlex reached up to 73.89%. Moreover, CONV exhibited the highest motility at pH 6.6 and 7.0 during the AD, stage while M - Zlex was the most motile at only pH 6.6. The motility in PT of CONV was high in pH 6.6, 7.0, and 7.4, whereas pH 6.6 and 7.0 are suitable for M - Zlex because weakly acidic solutions have an effect on stimulating X-sperm motility. Furthermore, the extenders osmolarity was in an appropriate range for sperm motility.
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