Influence of Light Durations and Sucrose Concentrations on Growth and Development of <I>Globba</> spp. under <I>In Vitro</I>
Article Sidebar
Main Article Content
Abstract
Globba is an important commercial flower of Thailand. It is popular cut flowers to worship during Buddhist Lent or other festivals. Globba flowers come in various colors, including purple, white, and pink. However, flowers can be cultivated as potted plants or used to decorate buildings and sites. The objective of this research was to determine the optimal light duration in combination with sucrose concentrations for the growth of Globba under in vitro conditions. The experiments utilized a 2x5 factorial in a completely randomized design, with factor A representing the influence of light durations from fluorescent light intensity of 3,000 Lux (8 and 16 hr/day) and factor B representing the concentrations of sucrose (0, 30, 40, 50, and 60 g/L). This research was conducted under 25±2 °C in vitro for 5 weeks. The results showed that no significant difference was found between light durations of 8 and 16 hr/day, while sucrose concentration were significantly different (P<0.01), The influence of light duration of 8 and 16 hr/day, when supplemented with 30 g/L sucrose, showed significantly different (P<0.01) compared to other treatments. However, interaction between light durations of 16 hr/day supplemented with 30 g/L sucrose was more suitable for shoot growth, the number of leaves, plant height and root number than other treatments.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
Akhtar, A., D. Zink and P.B. Becker. 2000. Chromodomains are protein - RNA interaction modules. Nature 407(6802): 405 - 409.
Aulia, M., T. Nasution, W. Muslihatin, S.N. Patrialoka, I.P.E.W. Pratama, P.Y. Aisyah, N. Jadid, T.R. Antika and M. Shovitri. 2024. The synergistic application of sucrose and various LED light exposures to enhance the in vitro growth of Stevia rebaudiana (Bertoni). Open Agriculture 9(1), doi: 10.1515/opag-2022-0305.
Boerio-Goates, J. 1991. Heat-capacity measurements and thermodynamic functions of crystalline α-D-glucose at temperatures from 10K to 340K. The Journal of Chemical Thermodynamics 23(5): 403 - 409.
Bonga, J.M. and P. Von Aderkas. 1992. In Vitro Culture of Trees. Dordrecht Kluwer Academic Publishers, Chicago. 236 p.
Huché-Thélier, L., L. Crespel, J. Gourrierec, P. Morel, S. Sakr and N. Leduc. 2016. Light signaling and plant responses to blue and UV radiations-perspectives for applications in horticulture. Environmental and Experimental Botany 121: 22 - 38.
Iwamoto, A., S. Ishigooka, L. Cao and L.P.R. De Craene. 2020. Floral development reveals the existence of a fifth staminode on the labellum of basal Globbeae. Frontiers in Ecology and Evolution 8: 1 - 16.
Jaimakaew, C., S. Thumdee and C. Sotthikul. 2016. Effects of sucrose, activated carbon, and light duration on in vitro microrhizome formation of ginger. Journal of Agriculture 32(1): 9 - 17. (in Thai)
Jo, E.A., R.K. Tewari, E.J. Hahn and K.Y. Paek. 2009. In vitro sucrose concentration affects growth and acclimatization of Alocasia amazonica plantlets. Plant Cell Tissue and Organ Culture 96: 307 - 315.
Kaveeta, L., M. Na Nakorn, S. Suwanawong, S. Tantiwiwat and N. Wongkantrakorn. 2017. Plant Physiology. Kasetsart University Publishing, Bangkok. 270 p. (in Thai)
Kim, S.J., E.J. Hahn, J.W. Heo and K.Y. Paek. 2004. Effects of LED on net photosynthetic rate, growth and leaf stomata of chrysanthemum plantlets in vitro. Scientia Horticulturae 101(1 - 2): 143 - 151.
Kongbangkerd, A. and S. Wannachart. 2006. Effect of light on in vitro seed germination and development of Dendrobium ochreatum Lindl. NU Science Journal 3(2): 231 - 241. (in Thai)
Kumyong, O. 2020. Study on rhizomes production of Globba (Globba spp.). M.S. Thesis. Maejo University, Chiang Mai. 139 p. (in Thai)
Kumyong, O. and P. Yangkhamman. 2020. Effect of sucrose on growth and development of Globba hybrida “MJ16” under in vitro conditions. Songklanakarin Journal of Plant Science 7(1): 2 - 9. (in Thai)
Mabberley, D.J. 2017. Mabberley’s Plant-book, 4th ed. Cambridge University Press, Cambridge. 1102 p.
Mahlberg, G.P. and S. Yarus. 1977. Effect of light, pH, temperature, and crowding on megaspore germination and sporophyte formation in Marsilea. Journal of Experimental Botany 28(5): 1137 - 1146.
Matgorzata, M. 2025. Effect of light spectrum, sucrose concentration, and 6-Benzyl-aminopurine on in vitro adventitious bulb formation in Tulipa tarda. Agronomy 15(3): 1 - 9, doi: 10.3390/agronomy15030642.
Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum 15(3): 473 - 497.
Nakdang, S., R. Chuengpanya, P.Sornyotha, A. Muangkroot, T. Jenjittikul and N. Chuenboonngarm. 2023. In vitro propagation of Curcuma lithophila Škorničk and Soonthornk an endemic species of Thailand with high potential to be used as ornamental plant. Journal of Applied Science and Emerging Technology 22(2): e248222, doi: 10.14416/JASET.KMUTNB.2023.02.010. (in Thai)
Nontalee, S., S. Saensouk and P. Saensouk. 2017. In vitro propagation of Globba annamensis Gagnep. for conservation of rare plant species in Thailand. Journal Khon Kean University Science 45(4): 858 - 872. (in Thai)
Ruamrungsri, S. 2015. Physiology of Flower Blub. Chiang Mai University Publishing. Chiang Mai. 276 p. (in Thai)
Sam, Y.Y. and H. Ibrahim. 2016. A new Globba with large white floral bracts from Peninsular Malaysia. PhytoKeys 73: 117 - 124.
Yuanjun, Y., Y. Zhou, J. Tan, L. Huang and Y. Xu. 2024. Globba Hongman, a cut-flower cultivar with red bract color and long vase life. HortScience 59(10): 1550 - 1552, doi: 10.21273/HORTSCI18113-24.
