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Kare Karon (Cymbidium aloifolium (L.) Sw.) is a wild epiphytic orchid that is at risk of extinction because of environmental changes and human behavior. The aims of this study were to investigate the effect of media on seeds germination and effect of growth regulators on protocorms growth of C.aloifolium. Six months old seeds derived from pod were cultured on MS medium supplemented with 3% sucrose and ND medium supplemented with 1% sucrose for 30 days. The results showed that ND medium was suitable for seed germination, as it had the highest germination percentage (100%) and germinated seeds developed into protocorms within 20-25 days. Fifty-day-old protocorms were cultured on ND medium supplemented with 0,0.5 and 1 mg/L NAA combined with 0, 1, 3, and 5 mg/L BA or Kinetin (Kn) for 90 days. Protocorms cultured on a medium with 1 mg/L BA showed the highest average number of shoot (7.27 shoot number/protocorm). The highest average number of roots/shoot was 3.40 and the maximum seedling height was 12.47 cm were found on medium supplemented with 1 mg/L NAA and 5 mg/L Kn. The seedlings were transferred to pots and grown in greenhouse condition for 30 days. The results showed that the highest survival percentage was 83 when transplanted to pots containing coconut husk chops. The data obtained from this study can be applied
for multiplication and conservation of this orchid species.