Genetic Diversity of Hemerodromia (Diptera: Empididae) in Loei Province

Abstract

Genus Hemerodromiais an index indicating the abundance of environments. This study aimed to study the genetic diversity of the Hemerodromia (Diptera: Empididae) in Loei province and assess the effectiveness of the DNA barcode genetic markers for identifying the species of Hemerodromia. Ninety adults of specimens were sampled from seven areas in Loei province by sweep net swung plane, up and down near bush areas around the waterfall or stream bank which have a clean environment and without human disturbance. Specimens were used for steps of DNA extraction, polymerase chain reaction, purification sent for sequencing and analyzing the genetic variation. The results showed that Hemerodromiain Loei province has seven species. DNA barcoding identification analysis based on the best close match method revealed good performance, with 100% of specimens correctly identified. A
phylogenetic tree based on the mitochondrial barcode sequences revealed that all of Hemerodromia species were monophyletic form clades. This basic research data will be useful for applying in further study in other organisms.