Effect of egg yolk powder on the quality and motility characteristics of post thawed cryopreserved bull semen
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Abstract
This study was carried out to evaluate the quality and motility characteristics of post-thawed cryopreserved bull semen in an extender containing egg-yolk tris glycerol with either fresh egg yolk or egg yolk powder (EP). Bull semen from four bulls was collected four times, once a week, and divided into three portions for three extenders. Group 1: semen was cryopreserved in egg-yolk tris glycerol (ETG) with 20 % fresh egg yolk (FEY); Group 2: semen was cryopreserved in ETG extender containing 10 % egg yolk powder (10EP); Group 3: semen was cryopreserved in ETG extender containing 15 % egg yolk powder (15EP). The diluted semen was filled and sealed into 0.25 ml French straws. Thereafter, the frozen semen straws were placed in liquid nitrogen. Thawed semen samples, after being kept for 24 hours, were analyzed for motility, progressiveness, sperm kinetics, and abnormal sperm using CASA. The results showed that the percentage of progressive motility from ETG was higher than that from 10EP and 15EP (9.47 %, 4.48 %, and 4.36 %, respectively; P<0.01). Average path velocity (VAP), straight line velocity (VSL), and curvilinear velocity (VCL) from ETG were higher than those from 10EP and 15EP (P<0.01). There was no significant difference in motility and abnormal sperm of bull semen cryopreserved in ETG, 10EP, and 15EP (P>0.05). These results indicate that 10 % and 15 % egg yolk powder in egg-yolk tris extender do not improve semen quality, specifically the percentage of progressive motility and velocity, of frozen-thawed bull semen.
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References
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