Effect of Dietary Supplemented with Musa ABB CV. Kluai “Namwa” on Immune Response and Disease Resistance Against Streptococcus agalactiae in Nile Tilapia

Abstract

This study focused on effects of Musa ABB CV. Kluai “Namwa” supplemented in pellet feeds on immune response and disease resistance against Streptococcus agalactiae in Nile Tilapia. Fish (average 162.82 ± 1.14 g) were manipulated into 4 groups. The supplemented fish were fed with pellet feeds supplemented with 0 (control), 1.0, 5.0 and 10.0 % (W/W) Kluai “Namwa” for 8 weeks. The result showed that supplementation of Kluai “Namwa” in the diet had some effected in hematological values in Nile tilapia (P>0.05). There was not significantly difference in the amount of white blood cells, haematocrit index and lysozyme activity which showed no significantly different in all fish group (P>0.05). However, the use of Kluai “Namwa” supplemented in the diet had effected on immune response of Nile tilapia, the values of nitroblue tetrazolium activity and superoxide dismutase had higher than the control group (P<0.05). In vivo, disease resistance against S. agalactiae was by intraperitoneally (i.p.) injection with 1 X 108 CFU/ml at 0.1 ml per fish. The supplementation fish fed with
Kluai “Namwa” in the diet had higher survival rate and relative percentage survival than the control group (P<0.05). The fish fed with 10.0 % diet had survival rate as 65.55 ± 3.85 and relative percentage survival as 50 %, followed by the group fed with 5.0 and 1.0 %, respectively. While, the lowest survival rate was show in the control group. The results indicated that dietary Kluai “Namwa” supplementation could enhance efficiency the immune response and disease resistance of S. agalactiae against of Nile tilapia.