Antioxidant Activity of Mixture Herbal Oil from Siamese Crocodile Oil (Crocodylus siamensis) Turmerica(Curcuma longa), Black Galingale (Kaempferia parviflora) and Plai (Zingiber cassumunar Roxb)

Abstract

The 3 formulas of crocodile oil mixture with Thai medicinal plants Zingiber cassumunar Roxb (Phlai), Curcuma longa (Turmeric) and Kaempferia parviflora (Black galingale) were developed as an alternative to skincare products. The study characterized by gas chromatography, Crocodile oil contained high concentration of palmitic acid, stearic acid, oleic acid and linoleic acid, the mixture oil (3 formulas) contain same high sabinene, sabinene hydrate, and (E)-1-(3,4 -dimethoxyphenyl) butadiene(DMPBD). The study shed light on 4 methods consists of phenolic content test, flavonoid content test, antioxidant efficiency test, and inhibition lipid peroxidation test of the mixture oil (3 formulas). The result indicated that crocodile oil mixed with Phlai has highest phenolic content 35.250 ± 0.902 mg GAE/ml, the crocodile oil mixed with turmeric has phenolic content 35.188 ± 1.659 mg GAE/ml, and the crocodile oil mixed with black galingale has phenolic content 16.406 ± 0.797 mg GAE/ml. The result of flavonoid contents test indicated that crocodile oil mixed with Turmeric has highest flavonoid contents 5.676±0.644 mg QE/ml, crocodile oil mixed with Black galingale has flavonoid
contents 3.281±0.505 mg QE/ml and crocodile oil mixed with Phlai has flavonoid contents 3.275±1.132 mg QE/ml. The result of antioxidant efficiency by DPPH assay indicated that crocodile oil mixed with Phlai was highest 119.735 ± 1.058 mg TEAC/ml, crocodile oil mixed with turmeric has 64.797 ± 1.481 mg TEAC/ml, and crocodile oil mixed with Black galingale has 62.199 ± 0.581 mg TEAC/ml. The result of inhibition lipid peroxidation indicated that crocodile oil mixed with
Turmeric has highest 40%, crocodile oil mixed with Phlai has 22%, and crocodile oil mixed with Black galingale 8%. The study showed that the mixed crocodile oils and 3 herbal plants have useful fatty acids for skincare and high anti-oxidant
which can be developed to be massage oil and various relevant products in the future.