Effect of Medium Strength and Plant Growth Regulators on Micropropagation of Venus Flytrap (Dionaea muscipula)

Abstract

Venus flytrap (Dionaea muscipula) is a popular carnivorous plant among growers because of the special characteristics. The demands for ornamental purposes are getting higher but the conventional propagation is not effective enough to increase the population of Venus flytrap. Therefore, micropropagation of Venus flytrap is considered as useful method to produce large amount of Venus flytrap population within limited duration to serve market demands for both conservative and commercial purposes. Effect of medium strength and plant growth regulators concentration on micropropagation of Venus flytrap were investigated to specify an optimum medium strength and plant growth regulators concentration on micropropagation of Venus flytrap The shoots of Venus flytrap were cultured on 1/2Murashige and Skoog medium (MS) 1/3MS, and 1/4MS medium supplemented with benzyl adenine (BA) at concentrations of  0 and 0.5 mg./l including with Naphthalene acetic (NAA) at concentrations of 0 and 0.1 mg./l. After 8 weeks culture, the results showed that the plantlets cultured on 1/4MS medium supplemented with 0.5 benzyl adenine (BA) had the highest fresh weight, shoot size, and leaf length. However, the plantlets cultured on 1/3MS medium supplemented with 0.5 benzyl adenine (BA) had the highest shoot number.