Efficacy of acidic electrolyte water to pathogen inactivation

Abstract

The objective of this study was to optimize the conditions for producing the acidic electrolyte water by using electrolysis and to determine the chemical properties. Then, investigated the properties of acidic electrolyte water to disinfect pathogenic bacteria, including Gram positive bacterial Staphylococcus aureus and Gram negative bacteria Escherichia coli was performed. After that, the stability of the acidic electrolyte water was tested. The results showed that the optimum conditions for the production of the acidic electrolyte water were 1,000 mL of 3% NaCl added 15 mL of 5% vinegar and passed through electric current 12 volts for 5 min. The chemical properties of the acidic electrolyte water showed pH value of 3.29, strongly positive of oxidation reduction potential (ORP) of 1280 mV and available chlorine concentration (ACC) of 80 ppm. The results indicated that the acidic electrolyte water could be inactivated microbial due to its oxidizing properties. The acidic electrolyte water absolutely destroyed tested bacterial cells from the first 15 seconds. The stability tests indicated that the acidic electrolyte water were stable at room temperature in a sealed container for up to 4 weeks.