Efficacy of modified dietary supplement with local fruits on proliferation of Drosophila melanogaster larvae and adults

Abstract

The fruit fly (Drosophila melanogaster) is a useful animal model for studying genetic inheritance. Fruit fly cultivation in the laboratory room is essential. Each laboratory room, as well as the culture media, was developed using a variety of indigenous components. The purpose of this study was to compare the effect between the five modified medium supplement with different fruits, including ripe mango (T1), ripe papaya (T2), ripe pineapple (T3), ripe jackfruit rags (T4), ripe chempedak fruit rags (T5), and control (T0), for increasing the number of fruit fly larvae and adults. Our results indicated that the larvae and adults in the modified medium were significantly more numerous than those in the control group (p=0.00006 and p=0.00001, respectively). The modified T2 and T4 medium significantly increased the average number of larvae of 72.2±11.4 and 70.4±22.3 instar larvae and adult of 49.0±4.0 and 40.8±2.8 flies, respectively. In T2 and T4 medium, the fruit ratio was optimized between 2 and 8% (w/v). The maximum number of larvae (112.67±34.82) was seen in the T4–4% media, which contained 4% (w/v) ripe jackfruit rags. T2–6%, mixed with 6% (w/v) ripe papaya, and T4–4%, which had the higher adult population than other medium of 43.3±18.4 and 41.3±16.4 flies, respectively. In addition, T4–4% and T2–6% medium had not affect on reproduction and the growth of the offspring generation. The genetic inheritance of the F3–D. melanogaster generation fed with both modified mediums showed that the phenotypic and male–to–female ratio were not significantly different from the control group.