The potential of Cannabis sativa extract to inhibit pathogens in animals
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Abstract
This research was aimed to screen the antimicrobial activities of two varieties of Cannabis sativa (FoiThongphuphayon cv. and Hanggrarok Roei-Et cv.) leaf extracts against pathogens including bacteria causing mastitis in dairy cows and some yeasts and filamentous fungi isolated from dermatitis and respiratory disease affected animals. Crude extracts from the leaves of cannabis were obtained using extractions by various solvents including ethanol, methanol, acetone, and chloroform. Disc diffusion was used as an antimicrobial and antifungal activity screening method, and minimum inhibitory concentration (MIC) and minimum bacteriocidal/fungicidal concentration (MBC/MFC) were further measured using the micro broth dilution method. The methanol extracts from both varieties of cannabis were generally effective in inhibiting most of the tested bacterial strains including three gram-positive: Staphylococcus aureus A0527, Staphylococcus haemolyticus A12321, and Staphylococcus pneumonia 001.12, and four gram-negative bacteria: Pseudomonas aeruginosa A11015, Mycoplasma pneumoniae 001.1, Klebsiella pneumoniae 001.3 and Escherichia coli A24622 at 50 µg/ml MIC and 400 µg/ml MBC, except for Staphylococcus faecalis 001.9 resisting inhibition. The methanol extracts were also effective against the tested dermatitis-causing yeasts and fungus including Candida albican, Malassesia pachydermatis, Trichopyton sp., as well as the respiratory system-affecting fungus, Cryptococcus neoformans, at 50 µg/ml MIC and 400 µg/ml MBC. Thus, the extracts from Cannabis are promising and should be promoted to be used clinically for clinical usage as treatment agents against pathogens, and also contain the potential to be used in the pharmaceutical industry going forward.
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References
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